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Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions.
Membranes (Basel)
October 2024
Department of Medical Physics and Biophysics, University of Split School of Medicine, 21000 Split, Croatia.
Giant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method. However, there are a number of parameters that can influence the success of the procedure.
View Article and Find Full Text PDFLipid vesicle formation by encapsulation of SMALPs in surfactant-stabilised droplets.
Heliyon
September 2024
Biomimetics Group, Division of Mechatronics, Biostatistics and Sensors (MeBios), Department of Biosystems, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.
Understanding the intricate functions of membrane proteins is pivotal in cell biology and drug discovery. The composition of the cell membrane is highly complex, with different types of membrane proteins and lipid species. Hence, studying cellular membranes in a complexity-reduced context is important to enhance our understanding of the roles of these different elements.
View Article and Find Full Text PDFSeveral common methods of making vesicles (except an emulsion method) capture intended lipid ratios.
Biophys J
October 2024
Department of Chemistry, University of Washington, Seattle, Washington. Electronic address:
Article Synopsis
- Researchers use different methods to create giant unilamellar vesicles, but each method can produce vesicles with different amounts of lipids, even when using the same materials.
- They studied the lipid ratios in vesicles made by five common methods and found that some methods have small differences while one method, emulsion transfer, had a much lower amount of cholesterol than expected.
- The results suggest that small changes in lipid ratios can be really affected by the method used, but experiments that change lipid ratios more dramatically will be less affected by these variations.
Unlabelled: Researchers choose different methods of making giant unilamellar vesicles in order to satisfy different constraints of their experimental designs. A challenge of using a variety of methods is that each may produce vesicles of different lipid compositions, even if all vesicles are made from a common stock mixture. Here, we use mass spectrometry to investigate ratios of lipids in vesicles made by five common methods: electroformation on indium tin oxide slides, electroformation on platinum wires, gentle hydration, emulsion transfer, and extrusion.
View Article and Find Full Text PDFPoly(Sitosterol)-Based Hydrophobic Blocks in Amphiphilic Block Copolymers for the Assembly of Hybrid Vesicles.
Small
October 2024
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, Denmark.
Amphiphilic block copolymer and lipids can be assembled into hybrid vesicles (HVs), which are an alternative to liposomes and polymersomes. Block copolymers that have either poly(sitostryl methacrylate) or statistical copolymers of sitosteryl methacrylate and butyl methacrylate as the hydrophobic part and a poly(carboxyethyl acrylate) hydrophilic segment are synthesized and characterized. These block copolymers assemble into small HVs with soybean L-α-phosphatidylcholine (soyPC), confirmed by electron microscopy and small-angle X-ray scattering.
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