AI Article Synopsis
- The study compared the transformation rates of three silkworm strains, examining how effective a gene introduction method (using piggyBac vectors) was across them.
- While fertility rates were comparable across strains, the Nistari strain had a significantly higher transformation rate compared to the Golden-yellow-cocoon and Jiaqiu strains.
- The research suggests that genetic differences among silkworm strains, particularly the presence of natural piggyBac-like elements, could impact how well the gene introduction method works.
Article Abstract
The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence. Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258), the percentages of G(0) transformed moths in fertile moths and injected eggs were both significantly different (P=0.01368 and P=0.02398, respectively). The transformation rate of the Nistari strain (Indian strain) was significantly higher than that of the other two strains, Golden-yellow-cocoon (Vietnamese strain) and Jiaqiu (Chinese strain), which had similar rate. These results indicate that the transformation efficiency of the piggyBac-based system might vary with silkworm strains with different genetic backgrounds. The presence of endogenous piggyBac-like elements might be an important factor influencing the transformation efficiency of introduced piggyBac-derived vectors, and the diverse amount and activation in different silkworm strains might account for the significant differences.
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| http://dx.doi.org/10.1111/j.1745-7270.2007.00252.x | DOI Listing |
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