Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp. L2, and closest to Acinetobacter sp. DG880 [AY258108]. It could use phenanthrene as the sole carbon source. After 7 days culture, the degradation rate of phenanthrene was 96.3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Draft genome sequences of strain BW1 and sp. strain MK2 isolated from oil sands process-affected water.
Microbiol Resour Announc
December 2024
Department of Applied Microbial Ecology, Helmholtz Centre for Environmental Research UFZ, Leipzig, Germany.
Draft genomes of two phenanthrene-degrading bacterial isolates from oil sands process-affected water (OSPW) in Alberta, Canada were sequenced. Both isolates grew in close association on agar plates and were difficult to obtain axenically. They represent novel and sp.
View Article and Find Full Text PDFHigh phenanthrene degrading efficiency by different microbial compositions construction.
Front Microbiol
August 2024
Shaoxing Academy of Agricultural Sciences, Shaoxing, China.
Microbial remediation has become the most promising technical means for the remediation of polycyclic aromatic hydrocarbons (PAHs) non-point source contaminated soil due to its low cost of treatment, complete degradation of pollutants, and remediation. In this study, in order to demonstrate the phenanthrene degrading microbial diversity, phenanthrene was chosen as the representative of PAHs and strains capable of degrading phenanthrene were isolated and screened from the sedimentation sludge and the bottom sludge of oil tank trucks, and high throughput sequencing was used to check the dominant strains with a good degrading effect on phenanthrene. Results showed even more than 50% of phenanthrene was degraded in all samples, the composition of PAH-degrading bacteria was diverse, and different environments constructed different functional microbial groups, which resulted in the microbial adapting to the diversity of the environment.
View Article and Find Full Text PDFCharacterization of 2-phenanthroate:CoA ligase from the sulfate-reducing, phenanthrene-degrading enrichment culture TRIP.
Appl Environ Microbiol
October 2024
Environmental Microbiology and Biotechnology (EMB), Aquatic Microbiology, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Unlabelled: Polycyclic aromatic hydrocarbons (PAHs) are chemically stable pollutants that are poorly degraded by microorganisms in anoxic sediments. The anaerobic degradation pathway of PAHs such as phenanthrene starts with a carboxylation reaction forming phenanthroic acid. In this study, we identified and characterized the next enzyme in the pathway, the 2-phenanthroate:CoA ligase involved in the ATP-dependent formation of 2-phenanthroyl-CoA from cell-free extracts of the sulfate-reducing enrichment culture TRIP grown anaerobically with phenanthrene.
View Article and Find Full Text PDFMicrobial consortium assembly and functional analysis via isotope labelling and single-cell manipulation of polycyclic aromatic hydrocarbon degraders.
ISME J
January 2024
State Key Laboratory of Organic Geochemistry and Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
Article Synopsis
- Soil microbial flora is complex and diverse, making it hard to cultivate and understand its metabolic functions in natural settings.
- The study introduces a novel method for creating functional microbial consortia (FMCs) using phenanthrene to investigate biodegradation, employing advanced techniques for identifying and sorting specific bacteria.
- Results show that the newly formed FMCs have superior phenanthrene degradation capabilities in real soil compared to traditional methods, revealing insights into constructing effective consortia based on single-cell analysis and genetic pathways.
Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures.
Sci Rep
May 2024
Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Hydrocarbon contamination, including contamination with polycyclic aromatic hydrocarbons (PAHs), is a major concern in Antarctica due to the toxicity, recalcitrance and persistence of these compounds. Under the Antarctic Treaty, nonindigenous species are not permitted for use in bioremediation at polluted sites in the Antarctic region. In this study, three bacterial consortia (C13, C15, and C23) were isolated from Antarctic soils for phenanthrene degradation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!