Somatic embryoids obtained from cell suspension cultures of fennel in Linsmaier-Skoog medium containing 2,4-D and kinetin readily developed into plantlets when plated on a hormone-free agar medium. These plants were transplanted to the field to be tested for the uniformity of the chemically as well as the morphologically important characteristics of fruits. The results of field trials conducted for two years have confirmed that the clonal plants derived from somatic embryoids are remarkably uniform in all the characteristics examined in comparison with the control plants propagated through seeds. It is suggested, therefore, that the quality control of fennel fruits used for spice or medicine could be achieved by means of clonal propagation through somatic embryoids.
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http://dx.doi.org/10.1055/s-2006-962631 | DOI Listing |
Biol Methods Protoc
December 2024
Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC), Selektsionnaya St, 14, VNIISSOK, Odintsovo Reg., 143072 Moscow, Russia.
In this protocol for obtaining doubled haploids plants (DH), we propose a new method for microspore isolation. This method is useful for genotypes of the Brassicaceae family with low responsiveness to DH technology. For such crops, it allows increasing the embryo yield several times and sometimes obtaining embryos for the first time.
View Article and Find Full Text PDFBiol Methods Protoc
March 2024
The University of Queensland Frazer Institute, The University of Queensland, Brisbane, QLD 4102, Australia.
Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs , as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.
View Article and Find Full Text PDFZhejiang Da Xue Xue Bao Yi Xue Ban
April 2024
Department of Cardiology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
Induced pluripotent stem cells (iPSCs) are obtained by introducing exogenous genes or adding chemicals to the culture medium to induce somatic cell differentiation. Similarly to embryonic stem cells, iPSCs have the ability to differentiate into all three embryonic cell lines. iPSCs can differentiate into cardiac muscle cells through two-dimensional differentiation methods such as monolayer cell culture and co-culture, or through embryoid body and scaffold-based three-dimensional differentiation methods.
View Article and Find Full Text PDFPlants (Basel)
December 2023
Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Saffron ( L.), being one of the distinguished commercial spice crops in the world, is in demand for its culinary, colorant, and pharmaceutical benefits. In this study, a novel indirect somatic embryogenesis (SE) system was, thus, established for the study of this plant.
View Article and Find Full Text PDFIn Vitro Cell Dev Biol Anim
January 2024
Stem Cell Research Centre, Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, India.
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