The objective of this work was to study the inhibitory effects of antisense peptide nucleic acids (PNAs) targeted to domain II of 23S rRNA on bacterial translation and growth. In this paper, we report that PNA(G1138) or peptide-PNA(G1138) targeted to domain II of 23S rRNA can inhibit both translation in vitro (in a cell-free translation system) and bacterial growth in vivo. The inhibitory concentration (IC50) and the minimum inhibiting concentration (MIC) are 0.15 and 10 microM, respectively. The inhibition effect of PNA(G1138) in vitro is somewhat lower than that of tetracycline (IC50 = 0.12 microM), but the MIC of peptide-PNA(G1138) against Escherichia coli is significantly higher than that of tetracycline (MIC = 4 microM). Further studies based on similar colony-forming unit (CFU) assays showed that peptide-PNA(G1138) at 10 microM is bactericidal, but the bactericidal effect is less effective than that of tetracycline. Nevertheless, the results demonstrated that the peptide-PNA(G1138) treatment is bactericidal in a dose- and sequence-dependent manner and that the G1138 site of 23S rRNA is a possible sequence target for designing novel PNA-based antibiotics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/psc.835 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Determination of Gene Mutations Associated with Macrolide and Fluoroquinolone Resistance in Patients Infected with Mycoplasma genitalium].
Mikrobiyol Bul
October 2024
Hacettepe University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Ankara, Türkiye.
A sexually transmitted bacterium, Mycoplasma genitalium has varying rates of reported resistance to macrolide and some fluoroquinolone group antimicrobials recommended for the treatment of its infections. It is currently recommended that the treatment of these must be planned according to macrolide resistance status. The aim of this study was to determine the presence of macrolide resistance associated mutations (MRM) and fluoroquinolone resistance associated mutations (QRM) in patients infected with M.
View Article and Find Full Text PDFMolecular characterization of two cyanobacterial generitypes from their type localities in Scandinavia.
J Phycol
December 2024
Faculty of Science, Department of Botany, University of South Bohemia, České Budějovice, Czech Republic.
Over 400 cyanobacterial genera have been described up to the present. Since the Cambridge Rules (https://www.iapt-taxon.
View Article and Find Full Text PDFA highly prevalent and specific cryptic plasmid pBI143 for human fecal pollution tracking in a subtropical urban river.
Water Res
December 2024
CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia. Electronic address:
Microbial source tracking (MST) is a critical tool for identifying sources of human and animal fecal pollution in aquatic environments. To enhance human fecal pollution tracking, this study evaluated the performance characteristics of pBI143, a cryptic plasmid recently identified for potential MST applications. Nucleic acid samples from ten animal species were screened for pBI143, revealing its presence in a small number of pigs, cows, dogs, cats, and flying fox fecal samples.
View Article and Find Full Text PDFTrends of Mycoplasma genitalium infections in Berlin, Germany, 2017-2023.
J Glob Antimicrob Resist
December 2024
Praxis Prenzlauer Berg, Berlin, Germany.
Objectives: The cell wall-less species Mycoplasma genitalium is a sexually transmitted pathogen with strong tendency to acquire resistance. Current knowledge about trends of resistance rates and differences between the risk population of men who have sex with men (MSM) and heterosexual patients as well as about circulating genotypes in both groups is limited.
Methods: Between August 2017 and December 2023, M.
Comparison of qPCR protocols for quantification of "Candidatus Saccharibacteria", belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA.
PLoS One
December 2024
Pediatric Clinical Research Center "Invernizzi", Department of Biomedical and Clinical Sciences, University of Milan, Milan, Italy.
Background: Candidate Phyla Radiation (CPR) is a large monophyletic group encompassing about 25% of bacterial diversity. Among CPR, "Candidatus Saccharibacteria" is one of the most clinically relevant phyla. Indeed, it is enriched in the oral microbiota of subjects suffering from immune-mediated disorders and it has been found to have immunomodulatory activities.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!