AI Article Synopsis
- This study improves methods for analyzing tumor cell membranes by focusing on membrane proteins from isolated ovarian tumor cells rather than just soluble proteins.
- Two extraction methods (detergent and organic solvent) are used on cell pellets, with the detergent method being more effective and compatible with advanced proteome analysis techniques.
- From the analysis of 20,000 ovarian tumor cells, 773 membrane proteins are identified, achieving 22% coverage of the membrane proteome in the SwissProt Human protein database.
Article Abstract
This work expands our tissue proteome capabilities from the analysis of soluble proteins in previous studies to the examination of membrane proteins within the pellets of enriched and selectively isolated tumor cells procured from microdissected tissue specimens. The pellets of targeted ovarian tumor cells are treated by two different membrane protein extraction methods, including the use of detergent and organic solvent. The detergent-based membrane protein preparation protocol not only extracts proteins effectively from cell pellets but also is compatible with subsequent proteome analysis using combined capillary isoelctric focusing/nano reversed-phase liquid chromatography separations coupled with nano electrospray ionization mass spectrometry. Among proteins identified from an amount of pellet equivalent to 20 000 cells, 773 proteins are predicted to contain one or more transmembrane domains, corresponding to 22% membrane proteome coverage within the SwissProt Human protein sequence entries.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ac061613i | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!