Objective: To establish a determination method of astragulin in Thesium chinese.
Method: RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times.
Result: The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location.
Conclusion: The method was validated to show convenient and reliable.
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