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Genomic microsatellite characterization and development of polymorphic microsatellites in Eospalax baileyi.
Sci Rep
January 2025
Key Laboratory of Grassland Ecosystem (Ministry of Education), Pratacultural College, Gansu Agricultural University, Lanzhou, 730070, China.
Microsatellite markers are cost-effective, rapid, efficient, and show great advantages in in large-sample kinship analysis and population structure studies. However, microsatellite loci are seriously underdeveloped in non-model organisms. The plateau zokor (Eospalax baileyi) is a key species living underground in the Tibetan Plateau, the effective management of which has long been challenging.
View Article and Find Full Text PDFParental Reconstruction from a Half-Sib Population of Stoneless Jujube Mill. Based on Individual Specific SNP Markers Using Multiplex PCR.
Plants (Basel)
November 2024
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
The selection of unique and individual-specific SNPs is important as compared with universal SNPs for individual identification. Therefore, the main significance of this research is the selection of specific SNPs in male parent and the identification of offspring with these specific SNPs in their genome by multiplex PCR, which is utilized for genotyping of 332 half-sib plants of .This cost-effective method makes as much as possible to utilize the same amount of each pair of various targeted loci primers.
View Article and Find Full Text PDFDevelopment of Microsatellite Markers from Transcriptome of and Their Application in Multiplex PCR Panels.
Animals (Basel)
November 2024
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
Article Synopsis
- Genetic markers, especially polyallelic microsatellite markers, are important for improving aquaculture and managing fish breeding and family lines sustainably.* -
- Researchers developed 100 highly polymorphic SSR markers through transcriptome analysis and created four multiplex PCR panels for effective parentage assignment in six fish families.* -
- The study showed high success rates (over 90%) in assigning offspring to parents using the multiplex panels, making this method a valuable and efficient tool for breeding and family management in aquaculture.*
Pedigree reconstruction based on genotype data in chickens.
Poult Sci
December 2024
State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China; Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; Sanya Institute of China Agricultural University, Hainan, 572025, China. Electronic address:
A reliable pedigree serves as the backbone of genetic evolution in domesticated animals, providing guidance for daily management and breeding strategies. However, in commercial chicken breeding, pedigree errors and omissions are common. The large-scale application of genomic selection provides an opportunity to reconstruct chicken pedigrees using SNP markers.
View Article and Find Full Text PDFKinship analysis and pedigree reconstruction by RAD sequencing in cattle.
GigaByte
July 2024
BGI Genomics, BGI Center, 9 Yunhua Road, Yantian District, Shenzhen, 518081, China.
Kinship and pedigree, used for estimating inbreeding, heritability, selection, and gene flow, are useful for breeding and animal conservation. However, as the size of crossbred populations increases, inaccurate generation and parentage assignment in livestock farms increase. Restriction-site-associated DNA sequencing is a cost-effective platform for single nucleotide polymorphism (SNP) discovery and genotyping.
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