AI Article Synopsis
- * A new, non-destructive image analysis method using the RGB color model was developed to estimate the content of betacyanines in the cells.
- * Treatment with 0.7 mM Triton X-100 for 15 minutes led to a 30% release of betacyanines, not compromising cell viability (over 70%), and resulted in cultures that regrew to 48% higher biomass after 14 days, with betacyanine levels just 25% lower than untreated cultures.
Article Abstract
The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (>or=70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.
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