A gentic instability in Phycomyces is described that appears to be associated with a single nuclear gene, dar. The wild type is able to take up riboflavin and its toxic analogue, deaza-riboflavin, from nanomolar concentrations in the medium. The mutants are unable to take up riboflavin and are resistant to deaza-riboflavin. Forward and reverse mutation rates are estimated to be 4 x 10(-5) and 2 x 10(-3) per nuclear division. Independently arisen dar mutants do not complement in heterokaryons. The mutant alleles are almost completely recessive. The phenotype of spores is not determined cell-autonomously, but is strongly influenced by the allele ratio among the nuclei in the sporangium of origin.
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| http://dx.doi.org/10.1093/genetics/92.1.27 | DOI Listing |
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