Spermicidal activity of extract from Cestrum parqui.

Objective: The leaf extract of Cestrum parqui was examined for its effects on sperm motility in vitro.

Methods: Washed sperm were prepared by discontinuous buoyant density gradient centrifugation and incubated with varying concentrations of extract from C. parqui (40-250 microg/mL) at 37 degrees C and 5% CO(2). Sperm motility and viability were assessed at different intervals ranging from 5 to 240 min. Morphological changes in human ejaculated spermatozoa after exposure to the extract were evaluated under transmission electron microscope.

Results: A dose- and time-dependent effect of this extract on sperm motility and viability was observed. The maximal spermicidal effect was observed with a 250-microg/mL dose of the extract. Transmission electron microscope revealed a significant damage to sperm membrane in both head and acrosomal membranes, notable swelling and disruption.

Conclusion: The present study indicates that this natural extract has potential spermicidal effect in vitro.