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A novel photoelectrochemical biosensor for sensitive detection of nucleic acids based on recombinase polymerase amplification and 3D-array titania nanorods.
Int J Biol Macromol
January 2025
NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine & The Second Affiliated Hospital, Hainan Medical University, Haikou 571199, PR China. Electronic address:
Nucleic acids detection is essential for diagnosing pathogens; however, traditional methods usually face challenges such as low sensitivity, lengthy reaction times, and strict temperature requirements. This study develops a novel photoelectrochemical (PEC) biosensor that integrates recombinase polymerase amplification (RPA) with a 3D-array titania (TiO) nanorods nanorod electrode, addressing the challenge of achieving sensitive detection of RPA-amplified nucleic acids products, thereby enabling earlier and more reliable pathogen detection. The biosensor utilizes a triple-binding mode involving FITC antibodies, target nucleic acids, and an HRP-streptavidin sandwich structure, significantly improving the bio-functionalization of the electrode surface.
View Article and Find Full Text PDFHistorical Overview of Tsutsugamushi Disease in Japan before World War II.
Infect Chemother
December 2024
Division of Infectious Diseases, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
Tsutsugamushi disease is a febrile mite-borne disease caused by . Before 1945, this disease had been prevalent in Niigata, Akita, and Yamagata prefectures for centuries, occurring in areas along major rivers in these prefectures every summer about a month after floods. The patients affected were farmers, possibly new settlers on reclaimed lands, who contracted the disease following bites of tiny red bugs.
View Article and Find Full Text PDFRe-evaluating Presumptive Treatment with Doxycycline/ Azithromycin in Children With Acute Febrile Illness in a Scrub Typhus Endemic Region: A Cross-Sectional Study.
Indian Pediatr
January 2025
ICMR Regional Medical Research Centre, Gorakhpur, Uttar Pradesh, India. Correspondence to: Dr Manoj Murhekar, Director-in-Charge, ICMR-Regional Medical Research Centre, Gorakhpur, Uttar Pradesh, India 273013.
Objective: To estimate the proportion of children with acute febrile illness (AFI) attending the peripheral health facilities in Gorakhpur, Uttar Pradesh, India, due to Orientia tsutsugamushi (Ots) and re-evaluate the strategy of presumptive administration of doxycycline/azithromycin (PDA) to patients with AFI.
Methods: Children aged 2-18 years with AFI attending 16 peripheral health facilities in Gorakhpur, Uttar Pradesh, were enrolled in September 2023. Blood samples were tested for O.
Abductor Palsy in Scrub Typhus.
Indian J Pediatr
December 2024
Department of Pediatrics, Gauhati Medical College, Guwahati, 781032, India.
Scrub typhus with urinary tract infection and leukopenia: A case series.
New Microbes New Infect
December 2024
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing City, China.
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