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Development and comprehensive greenness assessment of HPLC method for quality control of β-sitosterol in pharmaceutical ointments with trio-color coded evaluation.
BMC Chem
January 2025
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Elmessalah, Alexandria, 21521, Egypt.
A simple, rapid, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of β-sitosterol in the pharmaceutical dosage form of moist exposed burn ointment (MEBO). This method involved an effective sample procedure for extraction of β-sitosterol from MEBO using an alkali saponification agent composed of 0.8 N ethanolic NaOH and diethyl ether.
View Article and Find Full Text PDFThe eukaryotic genome is packaged into chromatin, which is composed of a nucleosomal filament that coils up to form more compact structures. Chromatin exists in two main forms: euchromatin, which is relatively decondensed and enriched in transcriptionally active genes, and heterochromatin, which is condensed and transcriptionally repressed . It is widely accepted that chromatin architecture modulates DNA accessibility, restricting the access of sequence-specific, gene-regulatory, transcription factors to the genome.
View Article and Find Full Text PDFUnveiling shadows: A data-driven insight on depression among Bangladeshi university students.
Heliyon
January 2025
Department of Computer Science and Engineering, BRAC University, Dhaka, Bangladesh.
Depression is more than just feeling sad. It is a severe and multifaceted mental health condition that impacts millions of individuals around the globe. Regrettably, it can even be more prevalent in university students of underdeveloped and developing countries like Bangladesh because of academic pressure, family and societal expectations, financial limitations, stigmatized social and cultural norms, unemployment concerns, lack of mental health awareness, etc.
View Article and Find Full Text PDFExploiting unique NP1 interface: Oriented immobilization via electrostatic and affinity interactions in a tailored PDA/PEI microenvironment enhanced by concanavalin A.
Talanta
January 2025
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211816, China. Electronic address:
Enzyme immobilization techniques are crucial for enhancing enzyme stability and catalytic efficiency. Traditional methods such as physical adsorption and simple covalent binding often fail to maintain enzyme activity and stability. In this study, an innovative multi-level immobilization strategy was proposed to achieve efficient targeted immobilization of nuclease P1 (NP1) by fine-tuning the surface microenvironment.
View Article and Find Full Text PDFA Simple One-Pot Method for the Synthesis of BiFeO/BiFeO Heterojunction for High-Performance Photocatalytic Degradation Applications.
Int J Mol Sci
December 2024
Joint International Research Laboratory of Information Display and Visualization, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.
This study presents a facile one-pot synthesis method to fabricate BiFeO-BiFeO-BiO heterojunction photocatalysts with controllable compositions and pure phases. Three different binary heterojunctions (BiFeO/BiFeO, BiFeO/BiO, and BiFeO/BiO) and a ternary BiFeO/BiFeO/BiO heterojunction were formed, all exhibiting significantly enhanced photocatalytic performance for the degradation of methylene blue (MB) and phenol under visible light irradiation, outperforming the individual compositions. Notably, the BiFeO/BiFeO heterojunction achieved the highest degradation efficiency (93.
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