A confirmation of Rexed's laminar hypothesis using the Sholl linear method complemented by nonparametric statistics.

Images of Golgi-impregnated neurons from laminae I to VI in the dorsal horn of the cat spinal cord were subjected to the linear Sholl analysis of concentric circles to support Rexed's hypothesis on the laminar organization of spinal gray matter in mammals. Since Rexed's determination of the laminae is based upon size, location, and grouping of cell bodies, neglecting one of the principal morphologic attributes of the neuron-the dendritic tree, the purpose of the present study was to evaluate Rexed's hypothesis testing the structure of dendritic arborization patterns of neurons. The differences in the complexity of dendritic trees between the groups of neurons from different laminae were evaluated by nonparametric statistics. Data obtained using Sholl's method is not always subjected to complete statistical analysis. The problem becomes particularly apparent in the quantitative examination of dendritic structures. Our aim was also to perform a careful analysis of our data for normality, in order to choose the appropriate statistical method for data processing. In the linear Sholl analysis, it is important to properly represent and interpret the frequency functions. The objective of this study was also to investigate the problems of determining the frequency functions, plotting the corresponding lines of regression, and measuring the degree of fluctuation of experimental data points around these lines. The main result of our testing is a confirmation of Rexed's laminar scheme: we have proved that there are 6 out of 10 possible pairs of samples where one member significantly differs from the other, i.e. one lamina is significantly distinguishable from the other.