Background & Objective: The incidence of lung cancer is high at Xuanwei, Yunnan Province, at where the mortality rate of this disease in women is the highest in China. This study was to establish a Xuanwei woman lung adenocarcinoma cell line, and provide an in vitro experimental model for the study of preventing and treating lung cancer.
Methods: The cells derived from a surgical specimen of a woman patient with lung cancer were primarily cultured. The biological characteristics of the cell line were studied with light and electron microscopes, determination of doubling time and growth curve, culturing in soft agar, flow cytometry (FCM), chromosome and G-band detection, c-12 multiple tumor markers detection, and inoculation in mice.
Results: Morphologic study, proliferation dynamics, and invasive growth showed that the cultured cells have malignant characteristics. Their chromosome numbers ranged from 55 to 69, with a mode number of 60-63. The tumor formation rate in mice was 100% after axillary transplantation of the cells; the morphology of the tumor cells was similar to that of the pathologic specimen of the patient. The cell line was named XWLC-05.
Conclusion: According to the newest rules of establishing a cell line in vitro, XWLC-05 is proved to be a new cell line of human lung adenocarcinoma.
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