[Cytotoxic effects of three kinds of eye drops for Chinese herbal medicines on various cell lines in vitro].

Objective: To study the cytotoxic effects of three kinds of eye drops on human cancer cells and normal cells.

Methods: Cytotoxic effects of Yuxingcao Eye Drop (YXC), Gegensu Eye Drop (GGS) and Shuanghuanglian Eye Drop (SHL) on 6 cell lines (CNE2, Glc-82, HRPE, Fibro, 3T3 and ECV-304) were assayed using MTF method. And cytotoxic efficacy was evaluated using fifty percent of inhibitory concentration (IC50).

Results: IC50 of YXC to human cancer cells CNE2 and Glc-82 were (11.5 +/- 0.25) microl/ml and (24.0 +/- 0.8) microl/ml; to normal cells HRPE, Fibro, 3T3 and ECV-304 were (18.0 +/- 3.5) microl/ml, (52.0 +/- 14.0) microl/ml, (17.5 +/- 3.5) microl/ml and (17.5 +/- 1.3) microl/ml, respectively. IC50 of GGS to CNE2, Glc-82, ECV-304 cells were (9.8 +/- 2.3) microl/ml, (17.0 +/- 5.0) microl/ml and (10.5 +/- 0.95) microl/ml, respectively. Under the concentration of 100 microl/ml, the average survival rates of HRPE, Fibro and 3T3 cells were 60.0%, 87.8% and 58.2%, respectively. IC50 of SHL to CNE2, Glc-82 and HRPE cells were (18.9 +/- 5.0) microl/ ml, (23.9 +/- 0.6) micorl/ml and (113.9 +/- 25.6) microl/ml. At the concentration of 100 microl/ml, the average survival rates of Fibro, 3T3 and ECV-304 Cells were 89.6%, 77.2% and 74.7%.

Conclusion: YXC has evident cytotoxicities to human cancer cells and normal cells. While, GGS and SHL has not obvious cytotoxic effects to normal cells.