AI Article Synopsis
- A study investigated how toluene and alcohol vapors affect the breakdown of toluene in the blood, focusing on gene expression in rat livers.
- Wistar male rats were exposed to toluene and 1-butanol vapors both separately and together over a four-week period, with gene expression analyzed afterward.
- The results showed a significant increase in the gene expression of CYP3A2 in the group exposed to both vapors compared to those exposed to only toluene, while other CYP genes showed no significant changes.
Article Abstract
A reduction of the biological half life of toluene in blood in the simultaneous exposure to toluene and alcohol vapors has been reported. To clarify the cause of this reduction, gene expression of CYP mRNA in liver of rats exposed to bi-component organic vapors was investigated. Wistar male rats were repeatedly exposed to 500 ppm of toluene and 300 ppm of 1-butanol vapors individually and simultaneously by inhalation 6 hours a day, five days a week for 4 weeks. After the exposure, the rats were sacrificed and the livers were collected and homogenized. RNA was extracted from the livers, and gene expression of CYP mRNA was observed by reverse transcription-polymerase chain reaction (RT-PCR). The gene expression of CYP3A2 in the simultaneous exposure group was significantly higher than that in the toluene exposure group. However, there was no significant difference in that of CYP1A2, CYP2B1, CYP2C11, CYP2E1 and CYP4A1.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7888/juoeh.28.395 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The effect of hot air treatment on volatile compounds in nectarine fruit and the regulation of glycosidically bound compounds by sugar.
Plant Physiol Biochem
January 2025
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:
In order to investigate the impact of hot air (HA) treatment on the sugars and volatiles in postharvest nectarine fruit, nectarines were treated with HA at 40 °C for 4 h and stored at 1 °C for 35 days. Changes of sugars, free and glycosidically bound volatiles, β-glucosidase (β-Glu) activity, and the gene expression of UGT (UDP-glucosyltransferase) in nectarine fruit were determined. The results showed that compared with CK, HA treatment delayed the firmness decline of 48.
View Article and Find Full Text PDFMutagenicity, DNA Repair Gene Polymorphism, and Differentially Expressed Plasma Protein Fractions among Textile Dyeing Workers.
J Occup Environ Med
November 2024
Industrial Medicine and Occupational Health, Public Health and Community Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Objectives: This study aimed to assess mutagenicity biomarkers among Egyptian textile dyeing workers, their alteration with gene polymorphism, and the changes in plasma proteins' expression.
Methods: Using a detailed questionnaire, a comparative cross-sectional study was conducted on 212 workers (106 textile dyeing exposed group and 106 control group). CBMN-Cyt assay, ERCC2 gene polymorphism, and plasma protein fractions were analyzed in workers' blood samples.
Sex Affects Cognitive Outcomes in HIV-1 Tat Transgenic Mice: Role of CCR5.
ASN Neuro
January 2025
Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia, USA.
People living with HIV (PLWH) experience HIV-associated neurocognitive disorders (HAND), even though combination antiretroviral therapy (cART) suppresses HIV replication. HIV-1 transactivator of transcription (HIV-1 Tat) contributes to the development of HAND through neuroinflammatory and neurotoxic mechanisms. C-C chemokine 5 receptor (CCR5) is important in immune cell targeting and is a co-receptor for HIV viral entry into CD4+ cells.
View Article and Find Full Text PDFNovel Immune Response Evasion Strategy to Redose Adeno-associated Viral Vectors and Prolong Survival in Surfactant Protein-B Deficient Mice.
Am J Respir Cell Mol Biol
January 2025
Ottawa Hospital Research Institute & CHEO Research Institute, Pediatrics, Ottawa, Ontario, Canada.
Surfactant protein-B (SP-B) deficiency is a lethal neonatal respiratory disease with few therapeutic options. Gene therapy using adeno-associated viruses (AAV) to deliver human cDNA (AAV-hSPB) can improve survival in a mouse model of SP-B deficiency. However, the effect of this gene therapy wanes.
View Article and Find Full Text PDFCodon bias, nucleotide selection, and genome size predict in situ bacterial growth rate and transcription in rewetted soil.
Proc Natl Acad Sci U S A
January 2025
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550.
In soils, the first rain after a prolonged dry period represents a major pulse event impacting soil microbial community function, yet we lack a full understanding of the genomic traits associated with the microbial response to rewetting. Genomic traits such as codon usage bias and genome size have been linked to bacterial growth in soils-however, often through measurements in culture. Here, we used metagenome-assembled genomes (MAGs) with O-water stable isotope probing and metatranscriptomics to track genomic traits associated with growth and transcription of soil microorganisms over one week following rewetting of a grassland soil.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!