AI Article Synopsis
- The study focused on creating and testing lactate biosensors to measure lactate levels in pharmaceutical products using lactate oxidase embedded in a sol-gel film on a platinum electrode.
- The biosensor demonstrated a linear detection range of 5x10^(-5) to 5x10^(-3)M when paired with a sequential injection system, indicating its effectiveness in various concentrations.
- The enzyme showed stability, maintaining its activity for at least 30 days and allowing for around 700 measurements without a decrease in sensitivity.
Article Abstract
This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5x10(-5) to 5x10(-3)M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h(-1). The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.
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| http://dx.doi.org/10.1016/j.jpba.2006.11.027 | DOI Listing |
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