Microspectroscopy of the photosynthetic compartment of algae.

Photochem Photobiol

Istituto di Biofisica, Area della Ricerca CNR, Pisa, Italy.

Published: February 2007

AI Article Synopsis

  • We used microspectroscopy to analyze the pigment composition in algae from various taxonomic groups as well as higher plants.
  • We tested the effectiveness of this technique on lab cultures to distinguish between different species and phylogenetic groups using advanced data processing methods.
  • Our findings revealed three major evolutionary lineages of algae, which aligned with current endosymbiotic theories.

Article Abstract

We performed microspectroscopic evaluation of the pigment composition of the photosynthetic compartments of algae belonging to different taxonomic divisions and higher plants. The feasibility of microspectroscopy for discriminating among species and/or phylogenetic groups was tested on laboratory cultures. Gaussian bands decompositions and a fitting algorithm, together with fourth-derivative transformation of absorbance spectra, provided a reliable discrimination among chlorophylls a, b and c, phycobiliproteins and carotenoids. Comparative analysis of absorption spectra highlighted the evolutionary grouping of the algae into three main lineages in accordance with the most recent endosymbiotic theories.

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Source
http://dx.doi.org/10.1562/2006-01-11-ra-772DOI Listing

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