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Metal-organic frameworks encapsulating gold nanoclusters and carbon dots for ratiometric fluorescent detection of formaldehyde in real food samples, construction materials and indoor environments.
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Hebei Lansheng Bio-Tech Co, Ltd, Shijiazhuang, 052263, P. R. China.
A novel fluorescence sensing nanoplatform (CDs/AuNCs@ZIF-8) encapsulating carbon dots (CDs) and gold nanoclusters (AuNCs) within a zeolitic imidazolate framework-8 (ZIF-8) was developed for ratiometric detection of formaldehyde (FA) in the medium of hydroxylamine hydrochloride (NHOH·HCl). The nanoplatform exhibited pink fluorescence due to the aggregation-induced emission (AIE) effect of AuNCs and the internal filtration effect (IFE) between AuNCs and CDs. Upon reaction between NHOH·HCl and FA, a Schiff base formed via aldehyde-diamine condensation, releasing hydrochloric acid.
View Article and Find Full Text PDFAptamer-based fluorescence biosensor for rapid detection of chloramphenicol based on pyrene excimer switch.
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State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Chloramphenicol (CAP) is widely used in treating bacteria infection in animals and humans. However, the accumulation of CAP in food and environment caused serious health risk to human. Consequently, sensitive and selective detection of CAP is of great importance in environmental monitoring and food safety.
View Article and Find Full Text PDFSingle-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris.
J Vis Exp
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Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara; Neuroscience Research Institute, University of California, Santa Barbara.
The tardigrade Hypsibius exemplaris is an emerging model organism renowned for its ability to survive environmental extremes. To explore the molecular mechanisms and genetic basis of such extremotolerance, many studies rely on RNA-sequencing (RNA-seq), which can be performed on populations ranging from large cohorts to individual animals. Reverse transcription polymerase chain reaction (RT-PCR) and RNA interference (RNAi) are subsequently used to confirm RNA-seq findings and assess the genetic requirements for candidate genes, respectively.
View Article and Find Full Text PDFHepatitis B virus (HBV) remains a critical public health issue in low- and middle-income countries (LMICs), particularly among pregnant women in Nigeria. Routine screening using rapid diagnostic kits is common in antenatal care, yet the accuracy of these tests can vary. This study aimed to determine the seroprevalencwe of HBV among pregnant women who had previously undergone screening using rapid diagnostic kits at Obafemi Awolowo Teaching Hospital, Ilesa, Osun State, Nigeria, to assess the effectiveness of initial screening and identify any missed cases.
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