AI Article Synopsis
Article Abstract
Objective: To determine whether prolonged exercise by conditioned sled dogs affects urine concentrations of homovanillic acid (a metabolite of dopamine), vanillylmandelic acid (a metabolite of norepinephrine and epinephrine), and cortisol.
Animals: 24 conditioned Alaskan sled dogs (2 to 8.5 years old) that were in training for a multiday endurance race.
Procedures: Voided urine samples were collected from 4 groups of dogs (randomly selected from 54 dogs) after no exercise (control group; n = 6 dogs), completion of a 160km run (group A; 3), completion of a 420-km run (group B; 7), and completion of a 560-km run (group C; 6). Urine cortisol concentrations were determined by use of an immunoassay technique; urine vanillylmandelic acid and homovanillic acid concentrations were measured via high-performance liquid chromatography.
Results: Compared with the control group, urine cortisol concentration in groups A, B, and C was significantly different (5.33 x 10(4) +/- 2.62 x 10(4) microg/dL vs 1.04 x 10(4) +/- 2.31 x 10(5) microg/dL, 8.88 x 10(4) +/- 5.49 x 10(4) microg/dL, and 6.31 x 10(4) +/- 5.09 x 10(4) microg/dL, respectively). Urine homovanillic acid concentration did not differ among the 4 groups. Vanillylmandelic acid was not detected in any urine samples.
Conclusions And Clinical Relevance: Results indicated that prolonged exercise by sled dogs did not affect urine homovanillic acid concentration but did increase urinary cortisol secretion, which is indicative of adrenocortical stimulation. The apparent lack of vanillylmandelic acid in voided urine samples requires further investigation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2460/ajvr.68.1.107 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biopeptide-rich fermented hemp seeds: Boosting anti-inflammatory and immune responses through Lactiplantibacillus plantarum probiotic fermentation.
Int J Biol Macromol
December 2024
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 24341, South Korea; Future F Biotech Co., Ltd, Chuncheon 24341, South Korea. Electronic address:
Cannabis sativa L. (hemp) seeds are increasingly recognized as a promising food source rich in phytochemicals that support inflammatory and immunological reactions. This study investigates whether fermentation with Lactiplantibacillus plantarum can further enhance these functional properties, paving the way for hemp seeds to be developed into potent functional food ingredients.
View Article and Find Full Text PDFScreening study of hydroxytyrosol metabolites from in vitro fecal fermentation and their interaction with intestinal barrier repair receptor AhR.
J Food Sci
December 2024
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, China.
Olive oil polyphenol hydroxytyrosol (HT) significantly repairs intestinal barrier function, but its absorption in the stomach and small intestine is limited. The metabolites of unabsorbed HT that reach the colon are crucial, yet their effects on colonic microbiota and intestinal barrier repair remain unclear. This study utilized in vitro simulated digestion and colonic fecal fermentation to investigate HT's digestion and fermentation.
View Article and Find Full Text PDFTo investigate the effects of chronic exposure to the cardiotonic steroid digoxin on locomotor activity, anxiety, and brain tissue monoamine content in Zebrafish. In total 24 adult (3-5 months) wild-type experimentally naïve zebrafish (50 : 50 ratio of females to males) were housed in 4-L tanks, in groups of six animals per tank. Two μM Digoxin was maintained in half of the tanks for 7 days.
View Article and Find Full Text PDFLeveraging supported liquid extraction and automated workflow for high efficiency and high quality clinical LC-MS/MS method for quantification of urinary 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic acid (VMA) and homovanillic acid (HVA).
Clin Chim Acta
January 2025
Medical-Scientific Department, LifeLabs Medical Laboratory Services, Toronto, ON M9W 6J6, Canada. Electronic address:
Background And Aims: 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic acid (VMA) and homovanillic acid (HVA) are neurotransmitter metabolites used for the diagnosis and monitoring of neuroendocrine tumors. This study's objective was to apply automation to improve efficiency and quality of a liquid chromatography tandem mass spectrometry (LC-MS/MS) procedure for quantification of 5-HIAA, VMA and HVA in human urine.
Methods: 5-HIAA, VMA and HVA in urine samples were extracted with automated supported liquid extraction (SLE) and quantified by LC-MS/MS utilizing scheduled multiple reaction monitoring (MRM).
Serotonin and Effort-Based Decision-Making: Dissociating Behavioral Effects of 8-OH-DPAT and PCPA.
Physiol Res
November 2024
Laboratory of Neurophysiology of Memory, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
Effort-based decision-making is particularly relevant to psychiatric conditions where motivation deficits are prominent features. Despite its clinical significance, the neurochemical mechanisms of this cognitive process remain unclarified. This study explores the impact of serotonin synthesis inhibition (PCPA) and modulation of serotonin release and 5-HT1A receptor agonism (8-OH-DPAT) on effort-based decision-making in rats.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!