AI Article Synopsis
- The study aimed to explore the link between specific IL1B gene polymorphisms and gastric cancer risk in a Chinese population by analyzing seven SNPs.
- A multiplex genotyping method was developed to efficiently identify these SNPs in both gastric cancer patients and non-cancer outpatients, with statistical methods applied to evaluate the results.
- The research found significant differences in genotype frequencies for three specific SNPs, indicating their association with gastric cancer risk and demonstrating the effectiveness of the new genotyping method.
Article Abstract
Objectives: To investigate the association between IL1B polymorphisms and risk of gastric cancer in a Chinese population, seven SNPs in the IL1B gene were selected for this study.
Methods: A multiplex genotyping method, which is based on adapter ligation and allele-specific amplification, was established to type seven SNPs on the IL1B gene simultaneously. One hundred and forty-one non-cancer outpatients and 97 patients with gastric cancer were genotyped, and the relation between IL1B polymorphisms and gastric cancer was statistically analyzed.
Results: The seven SNPs were successfully typed and the results were consistent with those obtained by both Sanger's sequencing and PCR-RFLP. Handling with statistical analysis, we observed significantly different genotype frequencies of 0794C>T (chi(2)=6.11, P=0.05), 1274C>T (chi(2)=6.86, P=0.03) and 2143T>C (chi(2)=6.86, P=0.03) between patients and controls.
Conclusion: ALM-ASA is a potential method for multiplex SNP typing with a low consumption of genomic DNA. SNPs 0794C>T, 1274C>T, and 2143T>C are associated with gastric cancer.
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| http://dx.doi.org/10.1016/j.clinbiochem.2006.10.018 | DOI Listing |
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