According to recently published guidelines, microsatellite instability (MSI) testing of colorectal cancers may be clinically indicated on a significant proportion of all colorectal tumors. To date, nothing has been published regarding the reproducibility of MSI testing between laboratories. We present MSI quality control activities experience of a six center multinational consortium, as laboratories developed competency with MSI testing and interpretation. The aim of this paper is to share lessons learned and to describe the final concordance rates in scoring MSI markers within this consortium.
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http://dx.doi.org/10.3233/cbm-2006-21-202 | DOI Listing |
Protein Pept Lett
January 2025
Scientific Research Center, Beijing ChosenMed Clinical Laboratory Co., Ltd. Beijing100176, China.
Background: The role of Zona pellucida glycoprotein 3 (ZP3) is unclear in pancreatic adenocarcinoma (PAAD).
Objective: This study aimed to explore the role of ZP3 in PAAD.
Methods: A comparative analysis of ZP3 gene expression was performed to discern differences between various types of cancer and PAAD, leveraging data sourced from The Cancer Genome Atlas (TCGA).
Anal Chem
January 2025
Department of Pharmaceutical Biosciences, Spatial Mass Spectrometry, Science for Life Laboratory, Uppsala University, SE-75124 Uppsala ,Sweden.
Multiomics analysis of single tissue sections using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) provides comprehensive molecular insights. However, optimizing tissue sample preparation for MALDI-MSI to achieve high sensitivity and reproducibility for various biomolecules, such as lipids, -glycans, and tryptic peptides, presents a significant challenge. This study introduces a robust and reproducible protocol for the comprehensive sequential analysis of the latter molecules using MALDI-MSI in fresh-frozen rodent brain tissue samples.
View Article and Find Full Text PDFSensors (Basel)
December 2024
Group of Quality Assurance and Industrial Image Processing, Faculty of Mechanical Engineering, Technische Universität Ilmenau, Gustav-Kirchhoff-Platz 2, 98693 Ilmenau, Germany.
Multispectral imaging (MSI) enables the acquisition of spatial and spectral image-based information in one process. Spectral scene information can be used to determine the characteristics of materials based on reflection or absorption and thus their material compositions. This work focuses on so-called multi aperture imaging, which enables a simultaneous capture (snapshot) of spectrally selective and spatially resolved scene information.
View Article and Find Full Text PDFGenes (Basel)
November 2024
Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Shanghai 200032, China.
Background: Firstly, 5-hydroxytryptamine G-protein-coupled receptors () are a family of 13 genes associated with cancer progression. Nevertheless, a comprehensive understanding of in cancer remains largely lacking.
Method: We tested the gene expression levels and prognostic values for the in relation to pan-cancer.
BMC Public Health
January 2025
Vocational School of Health Services, Medical Services and Techniques Department, First and Emergency Aid Programme, Bayburt University, Bayburt, 69000, Turkey.
Aim: Identifying the risks of metabolic syndrome (MetS) can lead to early targeted interventions and thus contribute to improved quality of life by reducing the risk of developing MetS, diabetes or heart disease in the future. We aimed to develop a valid and reliable measurement tool to measure the MetS risk of the population.
Materials And Methods: In the methodological study, an item pool was created by reviewing the literature.
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