A new method for selective localization of flavan-3-ols in plant tissues involving glycolmethacrylate embedding and microwave irradiation.

Histochemistry

Institut für Pflanzenbau, Lehrstuhl für Obstbau, Technische Universität München, Federal Republic of Germany.

Published: December 1991

A method for selective staining of flavan-3-ols in plant tissues fixed with glutaraldehyde is given. The use of glycolmethacrylate as embedding medium allows the sulphuric acid-containing staining solution to be heated without destroying the fine structure of the tissue. The distribution of flavan-3-ols and proanthocyanidins in different plant tissues is discussed.

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http://dx.doi.org/10.1007/BF00266765DOI Listing

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