Background: The favorable properties of water-soluble CdTe nanocrystals as novel biological luminescent label over conventional fluorescent probes have attracted considerable interest. The magnetic separation technique has widely been applied to various aspects in biotechnology in recent years. In this paper, we made use of CdTe nanocrystals and dextran-Fe3O4 magnetic nanoparticles for fluorescence immunoassay.
Methods: The CdTe nanocrystals and dextran-Fe3O4 magnetic nanoparticles were applied to immunoassay for the determination of human immunoglobulin G (HIgG). A rabbit anti-HIgG antibody (primary antibody) was immobilized on magnetic nanoparticles, which was used as a solid support. A sheep anti-HIgG antibody (secondary antibody) was attached to the surface of the CdTe nanocrystals via electrostatic interaction. The immunoassay was based on a sandwich immunoreaction of primary antibody on the magnetic nanoparticles, HIgG (or serum sample), and the secondary antibody labeled with CdTe nanocrystals.
Results: The CdTe label was compared with a fluorescein isothiocyanate (FITC) label. The CdTe is an order of magnitude more sensitive than the FITC. The immunoassay method was applied to determining the HIgG in practical samples and the results obtained are in good agreement with those obtained by nephelometry.
Conclusion: This technique may be applied in many types of antibody-antigen system.
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http://dx.doi.org/10.1016/j.cca.2006.11.013 | DOI Listing |
Mikrochim Acta
December 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China.
A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto FeO@SiO and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address:
Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (HS) is produced during spoilage-related processes, abnormal HS levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S) and thus the quality of high-protein foods in the early stage of storage.
View Article and Find Full Text PDFBiomater Adv
March 2025
Department of Basic, Zhejiang Pharmaceutical University, Ningbo 315500, PR China. Electronic address:
The flexible surface and chemical compatibility of hydrogels render them particularly appealing for research and development in antibacterial materials. However, designing tough hydrogels with multiple antibacterial mechanisms simultaneously remains a challenge. Inspired by the human skin, a hydrogel with bacterial antifouling, detection, and inactivation functions has been prepared using zwitterionic [2-(methylacrylyl) ethyl] dimethyl-(3-propyl sulfonate) ammonium hydroxide (SBMA) as the matrix and cadmium telluride quantum dots functionalised with cysteamine (CA-CdTe QDs) as the filler through micelle copolymerisation technology, achieving the integration of multiple antimicrobial mechanisms.
View Article and Find Full Text PDFACS Appl Bio Mater
December 2024
Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361005, P. R. China.
Superoxide anion (O) is a highly reactive oxygen species (ROS) within tumor cells, and its abnormal concentrations can lead to various diseases such as cancer, inflammation, and premature aging disorders. Here, we obtained a series of bioluminescence resonance energy transfer (BRET) systems that can be used for sensitive and specific detection of O by varying the type and reaction time of quantum dots (QDs) and combining them with different concentrations of recombinant aequorin. Among them, the recombinant aequorin-conjugated CdTe/CdSe QDs had the highest conjugation efficiency as the Aeq-QD BRET sensor, which has a remarkable energy transfer efficiency of 35.
View Article and Find Full Text PDFFood Chem Toxicol
January 2025
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Suzhou, Jiangsu, 215163, PR China; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, 215163, PR China. Electronic address:
This paper aimed to investigate the role of surface modification on the potential interaction between CdTe quantum dots (QDs) and ABC transporters in doxorubicin-resistant lung cancer (A549/DOX) cells. For this purpose, CdTe QDs modified with -glutathione (GSH), -carboxy (COOH), and -amino (NH) were applied, with the former two QDs exhibiting negative potentials and the latter ones being positive. All the three QDs reduced cell viability in a concentration-dependent manner, with NH-CdTe QDs being more toxic.
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