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Phytochemicals Controlling Enterohemorrhagic (EHEC) Virulence-Current Knowledge of Their Mechanisms of Action.
Int J Mol Sci
January 2025
Department of Bacterial Molecular Genetics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Enterohemorrhagic (EHEC) is a common pathotype of that causes numerous outbreaks of foodborne illnesses. EHEC is a zoonotic pathogen that is transmitted from animals to humans. Ruminants, particularly cattle, are considered important reservoirs for virulent EHEC strains.
View Article and Find Full Text PDFEscherichia coli in diarrhoeic lambs: Prevalence, virulence and antibiotic resistance.
Pol J Vet Sci
September 2024
Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences (RAJUVAS), Rajasthan, India.
The present study aimed to detect the prevalence, virulence and antimicrobial resistance genes profile of Escherichia coli isolated from diarrhoeic lambs. A total of 61 faecal samples were collected from diarrhoeic lambs. The presence of various virulence and antimicrobial resistance genes in E.
View Article and Find Full Text PDF[Population genetics of enterohemorrhagic Escherichia coli using whole-genome sequencing analyses].
Nihon Saikingaku Zasshi
December 2024
Department of Bacteriology I, National Institute of Infectious Diseases.
Enterohemorrhagic Escherichia coli (EHEC) is an important pathogen since more than 3,000 cases have been reported annually in Japan. With the advent of next-generation sequencing, it has become feasible to analyze numerous strains using whole-genome sequence (WGS) analysis, making its application to surveillance a realistic possibility. In this paper, we introduce the following research outcomes achieved by our group utilizing WGS analysis of EHEC: 1) development of a WGS analysis pipeline to enhance the accuracy of the surveillance, 2) investigation of the dynamics of mobile elements such as plasmids and phages, and 3) analysis of the phylogeny and pathogenicity of newly identified highly pathogenic EHEC strains.
View Article and Find Full Text PDFHydrolysis probe assays for the detection of pathogenic Enterohemorrhagic Escherichia coli: Multi-Country validation study.
Food Res Int
November 2024
Department of Health, Nutrition and Food Sciences, Florida State University, Tallahassee, FL, USA. Electronic address:
Article Synopsis
- Enterohemorrhagic Escherichia coli (EHEC) strains, linked to red meat outbreaks, pose serious global food safety issues, especially since current detection methods often yield false positives due to non-pathogenic strains.
- This study focused on developing and validating four new hydrolysis probe assays specifically designed to detect pathogenic E. coli serogroups O26, O103, O111, and O121, showing an impressive accuracy of 94.8% in identifying virulent strains.
- The newly standardized assays offer reliable detection tools for food laboratories worldwide, helping to mitigate waste and financial losses from incorrect positive test results associated with harmless non-pathogenic E. coli.
Characterization of non-O157 enterohemorrhagic Escherichia coli isolated from different sources in Egypt.
BMC Microbiol
November 2024
Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Article Synopsis
- Enterohemorrhagic Escherichia coli (EHEC) O157 is known for causing serious illnesses like hemorrhagic colitis and hemolytic uremic syndrome, but non-O157 strains are also emerging as significant pathogens.
- In a study conducted in Egypt, 335 samples were collected, revealing that nearly half were EHEC, with the O111, O91, O26, and O55 serotypes being the most common across various sources like stool, urine, and food products.
- The analysis of virulence genes showed high prevalence of genes such as sheA, stx2, and eae, and genetic testing revealed diverse strains with significant similarities, highlighting the complexity and potential
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