AI Article Synopsis
- A study compared duplex RT-PCR and direct immunofluorescence assays (DFAs) for detecting influenza A and B viruses in nasal samples collected during 2001-2003, using virus isolation in kidney cells as a reference.
- Among 404 samples, 78 tested positive for influenza, with a high concordance rate of 96% across all tests.
- RT-PCR demonstrated 92.3% sensitivity and 98.5% specificity, while DFA showed 93.6% sensitivity and 97.2% specificity; both methods are advantageous for quick and efficient virus detection, especially in a pandemic context.
Article Abstract
A duplex reverse transcription polymerase chain reaction (RT-PCR) and direct immunofluorescence assays (DFAs) were evaluated for detection of influenza types A and B in comparison with virus isolation in Madin-Darbin canine kidney cells. Four hundred four nasal wash were collected from individuals presenting with acute respiratory symptoms during 2001 to 2003 influenza seasons. According to the reference method, 78 (19.3%) samples were infected by influenza virus: 46 were type A and 32 type B. The overall concordance between the 3 assays was 96%, with 317 negative and 71 positive samples in all tests. RT-PCR reached 92.3% sensitivity and 98.5% specificity, and for DFA, the corresponding values were 93.6% and 97.2%, respectively. DFA and RT-PCR could be applied in different routine settings, resulting as an advantage compared with virus isolation: DFA provides rapid results for clinical purposes, but RT-PCR allows running more samples, an important concern in early pandemic circumstances.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2006.11.004 | DOI Listing |
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