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Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus. | LitMetric

Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.

Appl Environ Microbiol

Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium.

Published: February 2007

AI Article Synopsis

Article Abstract

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828643PMC
http://dx.doi.org/10.1128/AEM.01873-06DOI Listing

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