A 1050 bp up-stream regulatory fragment of the transcription factor gene NAC1 in Arabidopsis thaliana was isolated using polymerase chain reaction (PCR) based techniques. The fragment was used to substitute the 35S promoter of the pBI121 plasmid to construct a beta-glucuronidase gene (GUS) expression system. The construct was introduced into tobacco (Nicotiana tabaccum) plants by the Agrobacterium-mediated transferring method. GUS expression pattern was studied by using the transgenic lines. The results showed that the GUS driven by the NAC1 up-stream regulatory region was specifically expressed in the root meristem region, basal areas of the lateral root primordium and the lateral roots. The GUS expression was induced by 3-indolebutyric acid (IBA) and gibberellins (GA3 and GA4+7). The results indicated that the up-stream regulatory fragment of NAC1 responded to plant hormones. The fragment might be involved in both auxins and gibberellins signaling in promoting the development of lateral roots.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11427-006-2021-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring gene expression signatures in preeclampsia and identifying hub genes through bioinformatic analysis.
Placenta
January 2025
Department of Pathology, College of Medicine, Qassim University, Buraidah, 51911, Saudi Arabia. Electronic address:
Introduction: Preeclampsia (PE) is a multisystem disease that affects women during the pregnancy. Its pathogenicity remains unclear, and no definitive screening test can predict its occurrence so far. The aim of this study is to identify the critical genes that are involved in the pathogenicity of PE by applying integrated bioinformatic methods and to investigate the genes' diagnostic capability.
View Article and Find Full Text PDFNeuropeptide Bursicon and its receptor-mediated the transition from summer-form to winter-form of .
Elife
November 2024
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
Seasonal polyphenism enables organisms to adapt to environmental challenges by increasing phenotypic diversity. exhibits remarkable seasonal polyphenism, specifically in the form of summer-form and winter-form, which have distinct morphological phenotypes. Previous research has shown that low temperature and the temperature receptor regulate the transition from summer-form to winter-form in by impacting cuticle content and thickness.
View Article and Find Full Text PDFNuclear receptor TLX functions to promote cancer stemness and EMT in prostate cancer via its direct transactivation of CD44 and stem cell-regulatory transcription factors.
Br J Cancer
November 2024
School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong, China.
Article Synopsis
- Prostate cancer stem cells (PCSCs) are important for therapy resistance and metastasis in castration-resistant prostate cancer (CRPC), and their regulation is not well understood.
- The orphan nuclear receptor TLX has been identified as a key player that promotes tumor growth by repressing the androgen receptor and triggering pathways that lead to cancer stemness and epithelial-mesenchymal transition (EMT).
- Research shows that TLX enhances the expression of genes associated with stemness and EMT, such as CD44, SOX2, POU5F1, and NANOG, making it a significant regulator in the progression and aggression of prostate cancer.
Genome-Wide Identification and Expression Analysis of the YTH Domain-Containing RNA-Binding Protein Family in .
Int J Mol Sci
May 2024
State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.
-methyladenosine (mA) is one of the most abundant chemical modifications on mRNA in eukaryotes. RNA-binding proteins containing the YT521-B (YTH) domain play crucial roles in post-transcriptional regulation of plant growth, development, and stress response by reading the mA mark. However, the YTH domain-containing RNA-binding protein family has not been studied in a valuable and medicinal tree such as () yet.
View Article and Find Full Text PDFInteraction of Calmodulin with TRPM: An Initiator of Channel Modulation.
Int J Mol Sci
October 2023
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo Namesti 2, 16000 Prague, Czech Republic.
Transient receptor potential melastatin (TRPM) channels, a subfamily of the TRP superfamily, constitute a diverse group of ion channels involved in mediating crucial cellular processes like calcium homeostasis. These channels exhibit complex regulation, and one of the key regulatory mechanisms involves their interaction with calmodulin (CaM), a cytosol ubiquitous calcium-binding protein. The association between TRPM channels and CaM relies on the presence of specific CaM-binding domains in the channel structure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!