A metaproteomics approach comprising two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) was applied to the largely uncultured infant fecal microbiota for the first time. The fecal microbial metaproteome profiles changed over time, and one protein spot contained a peptide sequence that showed high similarity to those of bifidobacterial transaldolases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828649 | PMC |
| http://dx.doi.org/10.1128/AEM.01921-06 | DOI Listing |
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