The need for low-cost DNA sequence detection in clinical applications is driving development of new technologies. We demonstrate a method for detection of mutations in a DNA sequence purely by electronic means, and without need for fluorescent labeling. Our method uses an array of nanopores to perform synchronized single-molecule force spectroscopy measurements over many molecules in parallel, yielding detailed information on the kinetics of hundreds of molecule dissociations in a single measurement.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1796826 | PMC |
| http://dx.doi.org/10.1529/biophysj.106.094060 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Photocatalytic Oxidative Coupling of Methane to Ethane Using CO2 as a Soft Oxidant Over the Au/TiO2-Vo Nanosheets.
Angew Chem Int Ed Engl
December 2024
University of Science and Technology of China, Hefei National Research Center for Physical Sciences at Microscale, jinzhai road, hefei, CHINA.
Herein, we first report a photocatalytic OCM using CO2 as a soft oxidant for C2H6 production under mild conditions, where an efficient photocatalyst with unique interface sites is constructed to facilitate CO2 adsorption and activation, while concurrently boosting CH4 dissociation. As a prototype, the Au quantum dots anchored on oxygen-deficient TiO2 nanosheets are fabricated, where the Au-Vo-Ti interface sites for CO2 adsorption and activation are collectively disclosed by in situ Kelvin probe force microscopy, quasi in situ X-ray photoelectron spectroscopy and theoretical calculations. Compared with single metal site, the Au-Vo-Ti interface sites exhibit the lower CO2 adsorption energy and decrease the energy barrier of the *CO2 hydrogenation step from 1.
View Article and Find Full Text PDFControllable Growth of Monolayer and Bilayer WSe by Liquid-Phase Precursor via Chemical Vapor Deposition for Photodetection.
Nanomaterials (Basel)
December 2024
School of Flexible Electronics (Future Technologies), Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, China.
Two-dimensional WSe nanosheets have received increasing attention due to their excellent optoelectronic properties. Solid precursors, such as WO and Se powders, have been extensively employed to grow WSe nanosheets by the chemical vapor deposition (CVD) method. However, the high melting point of WO results in heterogeneous nucleation sites and nonuniform growth of the WSe nanosheet.
View Article and Find Full Text PDFSelf-Assembled Lubricin (PRG-4)-Based Biomimetic Surface-Enhanced Raman Scattering Sensor for Direct Droplet Detection of Melamine in Undiluted Milk.
Biosensors (Basel)
December 2024
Department of Biochemistry and Chemistry, La Trobe University, Bundoora, VIC 3086, Australia.
Surface-enhanced Raman scattering (SERS) is a powerful optical sensing platform that amplifies the target signals by Raman scattering. Despite SERS enabling a meager detection limit, even at the single-molecule level, SERS also tends to equally enhance unwanted molecules due to the non-specific binding of noise molecules in clinical samples, which complicates its use in complex samples such as bodily fluids, environmental water, or food matrices. To address this, we developed a novel non-fouling biomimetic SERS sensor by self-assembling an anti-adhesive, anti-fouling, and size-selective Lubricin (LUB) coating on gold nanoparticle (AuNP) functionalized glass slide surfaces via a simple drop-casting method.
View Article and Find Full Text PDFPolyetheretherketone Double Functionalization with Bioactive Peptides Improves Human Osteoblast Response.
Biomimetics (Basel)
December 2024
Department of Industrial Engineering, University of Padova, Via Marzolo 9, 35131 Padova, Italy.
In recent years, the demand for orthopedic implants has surged due to increased life expectancy, necessitating the need for materials that better mimic the biomechanical properties of human bone. Traditional metal implants, despite their mechanical superiority and biocompatibility, often face challenges such as mismatched elastic modulus and ion release, leading to complications and implant failures. Polyetheretherketone (PEEK), a semi-crystalline polymer with an aromatic backbone, presents a promising alternative due to its adjustable elastic modulus and compatibility with bone tissue.
View Article and Find Full Text PDFSpectral Data Fusion From Handheld Laser-Induced Breakdown Spectroscopy (LIBS) and X-ray Fluorescence (XRF) Analyzers for Improved Detection of Cerium in a Simulated Dispersal Accident.
Appl Spectrosc
December 2024
Nuclear Mission Branch, Air Force Research Laboratory, Kirtland AFB, New Mexico, USA.
This work implements a mid-level data fusion methodology on spectral data from handheld X-ray fluorescence and laser-induced breakdown spectroscopy analyzers to quantify plutonium surrogate (CeO) contamination in soil samples for the first time. Spectral data from each analyzer were used independently to train supervised machine learning regressions to predict Ce concentration. Fused features from both data sets were then used to train the same models, comparing prediction performance by evaluating model precision and sensitivity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!