Rapid pulsed-field gel electrophoresis protocol for subtyping of Streptococcus suis serotype 2.

J Microbiol Methods

Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China.

Published: March 2007

A rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping of Streptococcus suis serotype 2 was developed and evaluated using 27 clinical isolates from 22 epidemiologically unrelated patients. Results were matched against antibiogram, virulence genotyping and multi locus sequence typing (MLST). PFGE appeared to be the most discriminatory with numerical index of discrimination (D) equal to 0.87.

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http://dx.doi.org/10.1016/j.mimet.2006.10.010DOI Listing

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