The authors suggest a method for hemopoietic tissue cell fractionation into populations enriched for various hemopoietic elements. Centrifugation in a three-layer density gradient of mixtures of ficoll and verografin (d1 = 1.03 g/cm3, d2 = 1.09 g/cm3, d3 = 1.1 g/cm3) resulted in three fractions. The first contained lymphoid cells, the second erythroid cells (89.1 +/- 7.3%), and the third consisted of white blood cells (78.5 +/- 6.3%).
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