Synthesis of perylene-labeled base-discriminating fluorescent (BDF) nucleoside and its fluorescence properties.

Nucleic Acids Symp Ser (Oxf)

Newcat Institute, School of Engineering, Nihon University, Tamura, Koriyama, Fukushima 963-8642, Japan.

Published: August 2007

We have synthesized perylene labeled BDF probe and its photophysical properties were explored. Perylene labeled oligonucleotide probe is capable of detecting cytosine from its target mismatch sequence by enhancement of fluorescence intensity.

Download full-text PDF

Source
http://dx.doi.org/10.1093/nass/nrl089DOI Listing

Publication Analysis

Top Keywords

perylene labeled
8
synthesis perylene-labeled
4
perylene-labeled base-discriminating
4
base-discriminating fluorescent
4
fluorescent bdf
4
bdf nucleoside
4
nucleoside fluorescence
4
fluorescence properties
4
properties synthesized
4
synthesized perylene
4

Similar Publications

MicroRNAs (miRNAs) have emerged as essential biomarkers for disease diagnosis, and several techniques are available to determine type 2 diabetes (T2D) relevant miRNAs. However, detecting circulating miRNAs can be challenging due to their small size, low abundance, and high sequence similarity, often requiring sensitive detection approaches combined with additional amplification processes. Laser-induced fluorescence (LIF) is a classic analytical method suitable for sensitively detecting trace amounts of nucleotide acid.

View Article and Find Full Text PDF

Polystyrene--poly(polyethylene glycol monomethyl ether acrylate) (PSt--PPEGA) copolymers featuring pyrene and perylene as the Förster resonance energy transfer (FRET) donor (denoted as D-BCP) and acceptor (denoted as A-BCP), respectively, were synthesized via the reversible addition and fragmentation chain transfer (RAFT) polymerization. These copolymers were then used to form DA-mixed micelles via a dialysis method. The micelles consisted of D-BCP (mole fraction = 0.

View Article and Find Full Text PDF

Self-Assembled Perylene Diimide (PDI) Nanowire Sensitized InO@MgInS S-Scheme Heterojunction as Photoelectrochemical Biosensing Platform for the Detection of CA15-3.

Anal Chem

August 2024

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.

Inorganic/organic heterojunctions show promising applications as high-performance sensing platforms for photoelectrochemical (PEC) immunosensors. This work reports constructing a PEC biosensor for CA15-3 based on a self-assembled perylene diimide (PDI) nanowire sensitized InO@MgInS S-scheme heterojunction platform. P-type semiconductor CuO nanoparticles were designed as a signal burst source and were used as immunoassay labels.

View Article and Find Full Text PDF

Exonuclease-catalyzed recycling and annular four-footed DNA walking amplification-assisted "on-off-super on" signal transitions for photoelectrochemical biosensing of kanamycin.

Biosens Bioelectron

February 2024

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, China. Electronic address:

Photoelectrochemical (PEC) biosensors have exhibited a promising potential for assays of a large variety of analytes; however, how to realize their low background-based "super on" signal output is still a great challenge. Herein, we report a novel multiple nucleic acid amplification-assisted "on-off-super on" signal transition mechanism for the PEC biosensing of kanamycin antibiotics. The biosensing platform was constructed on a perylene-3,4,9,10-tetracarboxylic dianhydride-based photoelectrode, and its strong photocurrent could be well inhibited by an anchored ferrocene (Fc)-labeled hairpin DNA to produce a low background signal.

View Article and Find Full Text PDF

A Protocol for Activated Bioorthogonal Fluorescence Labeling and Imaging of 4-Hydroxyphenylpyruvate Dioxygenase in Plants.

Angew Chem Int Ed Engl

November 2023

National Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, College of chemistry, Central China Normal University, 430079, Wuhan, P. R. China.

4-Hydroxyphenylpyruvate dioxygenase (HPPD) plays a crucial role in the synthesis of nutrients needed to maintain optimal plant growth. Its level is closely linked to the extent of abiotic stress experienced by plants. Moreover, it is also the target of commercial herbicides.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!