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Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides. | LitMetric
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Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.

Yoshio Saito Kazuo Hanawa Keigo Hayashi Kaori Motegi Akimitsu Okaoto Isao Saito

Nucleic Acids Symp Ser (Oxf)

NEWCAT Institue, School of Engineering, Nihon University and SORST, Japan Science and Tecnology Agency, Tamura, Koriyama, 963-8642, Japan.

Published: June 2007

We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.

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http://dx.doi.org/10.1093/nass/49.1.153DOI Listing

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