An efficient RNA template-directed photoligation of oligodeoxynucleotides (ODNs) via 5-carboxyvinyl-2'-deoxyuridine ((CV)U) was described. When ODN containing (CV)U at the 5' end was photoirradiated with ODN containing a pyrimidine base at the 3' end in the presence of template RNA, an efficient photoligation was observed. RNA template-directed photoligation of ODNs should provide a useful technique for sensitive and accurate RNA detection.
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| http://dx.doi.org/10.1093/nass/49.1.143 | DOI Listing |
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