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Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing. | LitMetric
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Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing.

Yoshio Saito Keiichiro Kanatani Yuji Ochi Akimitsu Okamoto Isao Saito

Nucleic Acids Symp Ser (Oxf)

NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.

Published: July 2007

We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.

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 Source
http://dx.doi.org/10.1093/nass/48.1.243DOI Listing

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