AI Article Synopsis
- Researchers synthesized fluorescently-labeled ribonucleoside 5'-triphosphates, attaching dyes like carboxyfluorescein (FAM) and carboxytetramethylrhodamine (TAMRA) at position 5.
- These substrates were then specifically incorporated into RNA using T7 transcription with unnatural base pairs involving unique purines and 2-oxo(1H)pyridine.
- This method of fluorescent labeling RNA fragments is expected to enhance research in nucleic acid chemistry, biology, and related technologies.
Article Abstract
Ribonucleoside 5'-triphosphates of fluorescently-labeled 2-oxo(1H)pyridines, in which carboxyfluorescein (FAM) or carboxytetramethylrhodamine (TAMRA) was attached at position 5, were chemically synthesized. These substrates were site-specifically incorporated into RNA by T7 transcription mediated by unnatural base pairs between 2-amino-6-(2-thienyl)purine and 2-oxo(1H)pyridine or 2-amino-6-(2-thiazolyl)purine and 2-oxo(1H)pyridine. This site-specific fluorescent labeling of RNA fragments will be a powerful tool for the fields of nucleic acid chemistry, biology, and technology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/nass/48.1.35 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Geochemical exploration of rare earth element resources in highland karstic bauxite deposits in the Sierra de Bahoruco, Pedernales Province, Southwestern Dominican Republic.
PLoS One
January 2025
Dirección General de Minería, República Dominicana.
This study investigates the geochemical characteristics of rare earth elements (REEs) in highland karstic bauxite deposits located in the Sierra de Bahoruco, Pedernales Province, Dominican Republic. These deposits, formed through intense weathering of volcanic material, represent a potentially valuable REE resource for the nation. Surface and subsurface soil samples were analyzed using portable X-ray fluorescence (pXRF) and a NixPro 2 color sensor validated with inductively coupled plasma optical emission spectrometry (ICP-OES).
View Article and Find Full Text PDFFlexible Site-Specific Labeling-Mediated Self-Assembly Sensor Based on Quantum Dots and LUMinescent AntiBody Sensor for Duplexed Detection of Antibodies.
ACS Sens
January 2025
Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China.
Over recent years, the LUMinescent AntiBody Sensor (LUMABS) system, utilizing bioluminescence resonance energy transfer (BRET), has emerged as a highly effective method for antibody detection. This system incorporates NanoLuc (Nluc) as the donor and fluorescent protein (FP) as the acceptor. However, the limited Stokes shift of FP poses a challenge, as it leads to significant spectral cross-talk between the excitation and emission spectra.
View Article and Find Full Text PDFDesign and in vitro anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease.
Nucleic Acids Res
January 2025
School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Copper compounds with artificial metallo-nuclease (AMN) activity are mechanistically unique compared to established metallodrugs. Here, we describe the development of a new dinuclear copper AMN, Cu2-BPL-C6 (BPL-C6 = bis-1,10-phenanthroline-carbon-6), prepared using click chemistry that demonstrates site-specific DNA recognition with low micromolar cleavage activity. The BPL-C6 ligand was designed to force two redox-active copper centres-central for enhancing AMN activity-to bind DNA, via two phenanthroline ligands separated by an aliphatic linker.
View Article and Find Full Text PDFLong oligos: direct chemical synthesis of genes with up to 1728 nucleotides.
Chem Sci
December 2024
Department of Chemistry,, and Health Research Institute, Michigan Technological University Houghton Michigan 49931 USA
The longest oligos that can be chemically synthesized are considered to be 200-mers. Here, we report direct synthesis of an 800-mer green fluorescent protein gene and a 1728-mer 29 DNA polymerase gene on an automated synthesizer. Key innovations that enabled this breakthrough include conducting the synthesis on a smooth surface rather than within the pores of traditional supports, and the use of the powerful catching-by-polymerization (CBP) method for isolating the full-length oligos from a complex mixture.
View Article and Find Full Text PDFA Single Bioorthogonal Reaction for Multiplex Cell Surface Protein Labeling.
J Am Chem Soc
January 2025
Institute of Molecular Physiology, Shenzhen Bay Laboratory, Shenzhen 518132, China.
Small-molecule fluorophores are invaluable tools for fluorescence imaging. However, means for their covalent conjugation to the target proteins limit applications in multicolor imaging. Here, we identify 2-[(alkylhio)(ryl)ethylene]alononitrile (TAMM) molecules reacting with 1,2-aminothiol at a labeling rate over 10 M s through detailed mechanistic investigation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!