A novel method for the synthesis of oligoribonucleotides has been developed by the use of 1-(2-cyanoethoxy)ethyl (CEE) as a 2'-hydroxy protecting group. The CEE group was introduced to the 2'-position of an N-acyl-3',5'-O-silyl protected ribonucleoside under acidic conditions. A combination of the use of N-acyl and 2'-O-CEE protecting groups enables a reliable and complete two step deprotection, first with NH3aq-EtOH, then with TBAF in THF, without cleavage of internucleotidic linkages.
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| http://dx.doi.org/10.1093/nass/48.1.9 | DOI Listing |
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