PCC's were isolated either by adsorption to DEAE-Sephadex A-50 (particle size 40-120 microns; Pharmacia Uppsala) or by adsorption to Molselect DEAE-50 (particle size 100-320 microns; Reanal Budapest) from human citrated plasma after separation of factor VIII by cryoprecipitation. The two products obtained (without heparin) were both compared by in vitro (NAPTT, TGt50) and in vivo (venous stasis) model in rats acc. to WESSLER) tests. The agent prepared by adsorption to DEAE-Sephadex A-50 showed a significantly higher thrombogenicity than that prepared by Molselect DEAE-50. ED50 values for thrombus formation to amounted 15 Factor IX U/kg for the first product and to 210 factor IX U/kg for the second.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Sequential diethylaminoethyl dextran-grafting and diethylaminoethyl modification improve the adsorption performance of anion exchangers.
J Chromatogr A
August 2024
Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address:
Ion exchangers with high adsorption capacity, fast mass transfer, and high salt-tolerance synchronously are highly desired for high-performance protein purification. Here, we propose a sequential diethylaminoethyl dextran-grafting and diethylaminoethyl chloride modification strategy to achieve high-performance anion exchangers. The advantages of the double-modification strategy lie in: (1) the introduction of diethylaminoethyl in the second modification has no diffusion limitation due to the small molecular size, thus a high ionic capacity; (2) the grafting ligands not only provide three-dimensional adsorption space for high adsorption capacitybut alsofacilitate surface diffusion of protein by chain delivery.
View Article and Find Full Text PDFAntiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
J Ethnopharmacol
September 2020
College of Animal Science and Technology, Hainan University, Haikou, 570228, PR China. Electronic address:
Ethnopharmacological Relevance: The dry overground parts of Pogostemon cablin (Blanco) Benth. is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever. Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
View Article and Find Full Text PDFProtein adsorption to poly(ethylenimine)-modified Sepharose FF: VIII: Impacts of surface ion-exchange groups at different polymer grafting densities.
J Chromatogr A
January 2020
Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address:
Our previous studies on protein adsorption to the anion-exchangers of poly(ethylenimine) (PEI)-grafted Sepharose FF found that both adsorption capacity and uptake rate of bovine serum albumin (BSA) increased greatly when the PEI grafting density reached over a critical ionic capacity (cIC) due to the 3D protein binding and occurrence of "chain delivery" of bound proteins. Moreover, by the investigation on the anion-exchangers of diethylaminoethyl (DEAE)-modified and DEAE-dextran-grafted Sepharose FF, we found the unique role of surface ligand in facilitating protein uptake kinetics on dextran-functionalized anion exchangers as the "transfer station" for the "chain delivery" of bound proteins. However, what would be the contribution of surface ligands on the transport of bound proteins on PEI chains at a wide range across cIC, particularly at the lower IC range where no "chain delivery" was present? We have thus designed this research to answer the question.
View Article and Find Full Text PDFEmerging technology for increasing glucosinolates in arugula and mustard greens.
J Environ Sci Health B
July 2017
a Division of Environmental Studies , College of Agriculture, Food Science, and Sustainable Systems, Kentucky State University, Frankfort , Kentucky , USA.
Two plant species, arugula (Eruca sativa) and mustard (Brassica juncea) were field-grown under four soil management practices: soil mixed with municipal sewage sludge (SS), soil mixed with horse manure (HM), soil mixed with chicken manure (CM), and no-mulch bare soil (NM) to investigate the impact of soil amendments on the concentration of glucosinolates (GSLs) in their shoots. GSLs, hydrophilic plant secondary metabolites in arugula and mustard were extracted using boiling methanol and separated by adsorption on sephadex ion exchange disposable pipette tips filled with DEAE, a weak base, with a net positive charge that exchange anions such as GSLs. Quantification of GSLs was based on inactivation of arugula and mustard myrosinase and liberation of the glucose moiety from the GSLs molecule by addition of standardized myrosinase (thioglucosidase) and spectrophotometric quantification of the liberated glucose moiety.
View Article and Find Full Text PDFGlucosinolates in collard greens grown under three soil management practices.
J Environ Sci Health B
June 2016
a Division of Environmental Studies, College of Agriculture, Food Science, and Sustainable Systems , Kentucky State University, Frankfort , Kentucky , USA.
Glucosinolates (GSLs, β-D-thioglucoside-N-hydroxysulfates) are polar compounds present in varying amounts in members of the Brassicaceae family. They suppress soil-borne pests due to the biofumigant properties of the highly toxic isothiocyanates present in Brassica vegetables. The objectives of this investigation were to: (1) assess variation in GSLs concentrations among collard plants grown under three soil management practices: sewage sludge (SS) mixed with native soil, chicken manure (CM) mixed with native soil, and no-mulch (NM) native soil, (2) quantify GSLs concentrations in collard roots, leaves, and stems at harvest for potential use of their crude extracts in plant protection, and (3) assess myrosinase activity in soil amended with CM and SS mixed with native soil.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!