FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco.

Biotechniques

Carnegie Institution, Stanford, CA 94305, USA.

Published: November 2006

Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.

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Source
http://dx.doi.org/10.2144/000112264DOI Listing

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