One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.
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http://dx.doi.org/10.1007/s00253-006-0741-7 | DOI Listing |
BMC Genomics
December 2024
Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, Murcia, Spain.
Background: Extracellular vesicles (EVs) are essential for cell-to-cell communication because they transport functionally active molecules, including proteins, RNA, and lipids, from secretory cells to nearby or distant target cells. Seminal plasma contains a large number of EVs (sEVs) that are phenotypically heterogeneous. The aim of the present study was to identify the RNA species contained in two subsets of porcine sEVs of different sizes, namely small sEVs (S-sEVs) and large sEVs (L-sEVs).
View Article and Find Full Text PDFSci Rep
December 2024
Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, Vienna, Austria.
The early microbial colonization of the porcine gut is an important priming factor for gut and immune development. Nevertheless, little is known about the composition of microbes that translocate into the ileo-cecal lymph nodes (ICLN) in the neonatal phase. This study aimed to characterize age- and nutrition-related changes in the metabolically active bacterial and fungal composition of the ICLN in suckling and newly weaned piglets.
View Article and Find Full Text PDFPrev Vet Med
December 2024
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States.
Antimicrobial resistance is considered a global One Health threat. Controlling selection pressure by reducing antibiotic use in livestock is a significant component of the response to this threat. The science concerning use and resistance is complicated and affected by time from antibiotic exposure, changing bacterial fitness, and varies by drug and pathogen.
View Article and Find Full Text PDFFront Microbiol
December 2024
Department of Animal Science and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United States.
Previous studies have suggested that porcine peritoneal macrophages (PPMs) are resistant to PRRSV infection, whereas porcine alveolar macrophages (PAMs) are highly susceptible. This contrast is intriguing, as both cell types belong to the same monocyte/macrophage family. The current study aimed to investigate the host factors contributing to the differing susceptibility of PPMs and PAMs to PRRSV infection.
View Article and Find Full Text PDFJ Microbiol Immunol Infect
December 2024
Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Research and Technical Center for Sustainable and Intelligent Swine Production, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan. Electronic address:
Background/purpose: Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen with a negative economic impact on the global swine industry. In 2019, a suspected highly pathogenic strain, NPUST-108-929/2019 (108-929), was isolated from a pig farm in Pingtung with an outbreak of high mortality and analyzed. The characteristics of PRRSV 108-929 have barely been studied.
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