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Evaluation of cattle inoculated with Babesia bovis clones adhesive in vitro to bovine brain endothelial cells. | LitMetric

AI Article Synopsis

  • The study compared the virulence of two Babesia bovis clones (virulent 2F8 and reduced virulence RAD) that either adhere or do not adhere to bovine brain endothelial cells, using Holstein cattle for experimentation.
  • Twenty Holstein cattle, grouped and inoculated with either adhesive or nonadhesive clones, all experienced a decrease in packed cell volume (PCV), with the nonadhesive 2F8 group showing the most significant drop and prolonged fever.
  • Despite differences in adhesive characteristics and the greater individual impact of virulent subpopulations on PCV, no significant differences in pathogenicity were found among the four tested subpopulations.

Article Abstract

A comparative assessment of the virulence of Babesia bovis clones that adhere or not to bovine brain endothelial cells was done using two clones of B. bovis: (1) a clone phenotypically characterized as virulent (2F8) and (2) a clone of reduced virulence (RAD). Of these subpopulations, we selected those that had adhesive characteristics (a) or nonadhesive characteristics (na) in cultured endothelial cells. Twenty Holstein cattle, 12 months of age or older, were used in this study, and these cattle were randomly assigned to five groups of four animals each. The clones and their respective subpopulations were inoculated via intramuscular injection at a 0.5 x 10(7) infected erythrocyte dosage. Group A was inoculated with aRAD, group B with naRAD, group C with a2F8, group D with na2F8, and group E remained as a control. All inoculated animals showed a decrease in the packed cell volume (PCV), with group D showing the largest decrease (39.53%) and longest time (7 days) with rectal temperature above 39.5 degrees C. Babesia was observed in stained blood smears from only six cattle. While the four parasite subpopulations were pathogenic, significant differences were not noted among them, despite that the subpopulations considered to be virulent caused the greatest reduction in PCV per individual.

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Source
http://dx.doi.org/10.1196/annals.1373.058DOI Listing

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