AI Article Synopsis
- A new photoacoustic flow cytometry was created for real-time detection of cells, nanoparticles, and contrast agents in living organisms.
- The system uses a near-infrared tunable laser to study the behavior of Indocyanine Green and gold nanorods in cancer cells within mouse ear vasculature.
- It shows promise for various applications like targeted cancer therapy, detection of melanoma, studying drug effectiveness, and understanding immune responses, with a sensitivity that can detect one cancer cell among ten million normal blood cells.
Article Abstract
A new photoacoustic flow cytometry was developed for real-time detection of circulating cells, nanoparticles, and contrast agents in vivo. Its capability, integrated with photothermal and optical clearing methods, was demonstrated using a near-infrared tunable laser to characterize the in vivo kinetics of Indocyanine Green alone and single cancer cells labeled with gold nanorods and Indocyanine Green in the vasculature of the mouse ear. In vivo applications are discussed, including selective nanophotothermolysis of metastatic squamous cells, label-free detection of melanoma cells, study of pharmokinetics, and immune response to apoptotic and necrotic cells, with potential translation to humans. The threshold sensitivity is estimated as one cancer cell in the background of 10(7) normal blood cells.
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| http://dx.doi.org/10.1364/ol.31.003623 | DOI Listing |
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