The paper comparatively assesses routine and new medical technologies (metachromatic urine test, polymerase chain reaction, chromatographic mass spectrometry, and enzyme immunoassay) for detection of Mycobacterium tuberculosis and tuberculosis antibodies. It is shown that molecular biological and immune assays may be used only for rapid diagnosis and screening, followed by a full-scale phthisiourological study as the detection of markers of Mycobacterium tuberculosis and tuberculosis antibodies cannot be a criterion for establishing renal tuberculosis. The authors consider that the conventional microbiological tests whose validity is beyond question in case of positive results are the most established and practice-tested canons of phthisiourological diagnosis.
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Sci Rep
January 2025
Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK, Canada.
Bovine tuberculosis (BTB) is an infectious disease of livestock and wildlife species that is caused by pathogenic members of the Mycobacterium tuberculosis complex such as Mycobacterium bovis. Due to the introduction of M. bovis-infected bison in the 1920s, BTB is now endemic in wood bison (Bison bison athabascae) population within the Wood Buffalo National Park (WBNP) in northern Canada.
View Article and Find Full Text PDFSci Rep
January 2025
Laboratorio de Interacciones Hospedero-Patógeno, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Tuberculosis is a global public health concern, and understanding Mycobacterium tuberculosis transmission routes and genetic diversity of M. tuberculosis is crucial for outbreak control. This study aimed to explore the genomic epidemiology and genetic diversity of M.
View Article and Find Full Text PDFJ Proteomics
January 2025
State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China. Electronic address:
Although the phosphorylation of serine (S), threonine (T), and tyrosine (Y) is well-established, arginine phosphorylation (pR) has recently garnered significant attention due to its crucial role in bacteria pathogenicity and stress response. Mycolicibacterium smegmatis, a nonpathogenic surrogate of Mycobacterium tuberculosis, serves as a model for studying mycobacterial pathogenesis. A recent proteomics study identified six pR proteins in M.
View Article and Find Full Text PDFInfect Genet Evol
January 2025
Agri-Food and Biosciences Institute, AFBI Stormont, Veterinary Sciences Division, Belfast, UK.
Mycobacterium bovis, the causative agent of animal tuberculosis, exhibits a broad host range - infecting, inducing pathology and transmitting from both bovine and wildlife hosts. Considerable effort has been extended to understanding the role wildlife may play in persistence and spread of infection. Infected cervids can spread infection to conspecifics and sympatric livestock as observed in the white-tailed deer (Odocoileus virginanus) population of Michigan, USA.
View Article and Find Full Text PDFClin Microbiol Infect
January 2025
Wits Diagnostics Innovation Hub, Health Sciences Research Office, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Objectives: Tongue swabs (TS) have shown potential for detecting Mycobacterium tuberculosis complex (MTBC) through downstream molecular testing. Analytical performance varies, depending on the processing protocol and the molecular test used. This study aimed to first investigate ease-of-use of TS collection in addition to acceptability by individuals being assessed for TB and second to determine the performance of self-collected TS on the Xpert MTB/RIF Ultra (Ultra) assay (Cepheid, Sunnyvale, CA, USA) for MTBC and Rifampicin (RIF) resistance detection.
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