Horizontal transfer and hypovirulence associated with double-stranded RNA in Beauveria bassiana.

Mycol Res

Universidade Federal do Paraná, Departamento de Patologia Básica, Rua Lourenco Mourão, 57 Seminário, Curitiba CEP 81531-990, Brazil.

Published: December 2006

Beauveria bassiana strains from different hosts and geographic origins were assayed for the presence of double-stranded RNA (dsRNA). Two of them (15.4%) showed extra bands, with approximately 4.0-3.5 kb and 2-0.7 kb, respectively, after electrophoretic separation of undigested nucleic acids. Virus-like particles were approximately 28-30 nm diam. The dsRNA was maintained after conidiogenesis (vertical transmission) and was transmitted horizontally by hyphal anastomosis. Strains purged of dsRNA obtained after cycloheximide treatment showed increased conidial production when compared with strains carrying dsRNA particles. Bioassays demonstrated hypovirulence associated with dsRNA. The mean mortality against the insect Euschistus heros was reduced in strains containing dsRNA when compared with the isogenic dsRNA-free ones.

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http://dx.doi.org/10.1016/j.mycres.2006.08.009DOI Listing

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