AI Article Synopsis
- The study investigates how calcium signaling in liver cells (hepatocytes) changes during the regeneration process after partial tissue loss.
- At specific time points after liver surgery, researchers found that the liver cells responded less effectively to signals that usually raise calcium levels, indicating a phase of desensitization.
- This desensitization involved a decrease in certain receptors and alterations in calcium release patterns, linking these changes to the cells moving into a growth phase of their cycle.
Article Abstract
Background/aims: During liver regeneration, a network of cytokines and growth factors interact with hepatocytes, helping to restore the liver mass and functions after partial tissue loss. Agonists that trigger Ca2+ signals in the liver contribute to this process, although little is known about calcium signalling during liver regeneration.
Results: We observed two phases in which the hepatocyte response to calcium-mobilising agonists was greatly reduced versus control cells at 24h and five days after partial hepatectomy. We found that both phases of hepatocyte desensitisation involved the down-regulation of cell surface receptors and the type II InsP3 receptor. Single cell studies with flash photolysis of caged InsP3 revealed that InsP3-mediated Ca2+ release was slower in regenerating hepatocytes at 24, 48 h and 5 days than in control cells. Also, the temporal pattern of vasopressin-elicited intracellular calcium oscillations studied on fura2-loaded cells was altered, with the duration of each Ca2+ peak being longer. Finally, we showed an association between hepatocyte desensitisation and progression through the cell cycle towards the S phase at 24 h after hepatectomy.
Conclusions: Our study supports the remodelling of hepatocyte calcium signalling during liver regeneration, and that this change is partly linked with cell cycle progression.
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| http://dx.doi.org/10.1016/j.jhep.2006.08.014 | DOI Listing |
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