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Ultrasensitive and high selectivity detection of fibrin using Y-shaped DNA-homing peptide doped probe on localized surface plasmon resonance platform.
Anal Chim Acta
January 2025
Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, China.
Background: Localized surface plasmon resonance (LSPR) sensor has drawn continuous attention to application of the detection of antibody, protein, virus, and bacteria. However, natural recognition molecules, such as antibody, which possess some properties, including low thermal stability, complicated operation and high price, uncontrollability of length and size and a tendency to accumulate easily on the surface of chip to reduce the sensitive of method. Furthermore, common blocking agents are not suitable for development of novel biosensors.
View Article and Find Full Text PDFIntramolecular distance-regulated G4 DNA enzymatic activity-based chromophotometric system for visual monitoring of diquat.
Anal Chim Acta
January 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:
Background: As global food production continues to surge, the widespread use of herbicides has also increased concurrently, posing challenges like health risks and environmental pollution. Traditional detection methods for pesticide residues, such as diquat (DQ), were hampered by limitations like high expenses, lengthy detection times and complex operations, restricting their practical application in rapid clinical diagnosis.
Results: In light of the pressing necessity for the identification of minute pesticide residues and the intrinsic constraints of small molecule analysis, a novel chromophotometric biosensor targeting small molecules was developed based on bi-epitopes on single antibody to immobilize two DQ-PAL, inhibiting the hybridization of DQ-PAL.
Deep eutectic solution elution assisted ligand affinity assay: A useful tool for the active coumarins screening from Fructus cnidii.
Anal Chim Acta
January 2025
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, 300070, Tianjin, China. Electronic address:
Background: Many of the ligand affinity analyses are presented in water environment, and the hydrophilic solution such as methanol is used for dissociating the bound compounds. The obtained dissociation solution needs to be concentrated for improving the sensitivity of the assay. However, it is not good for the analysis of hydrophobic and volatile compounds such as coumarins.
View Article and Find Full Text PDFImproved activity and stability of cellulase by immobilization on FeO nanoparticles functionalized with Reactive Red 120.
Int J Biol Macromol
January 2025
Faculty of Petroleum and Chemical Engineering, Razi University, Kermanshah, Iran. Electronic address:
Cellulase is extensively used in the biorefinery of cellulosic materials to fermentable sugars in bioethanol production. Application of cellulase in the free form has disadvantages in enzyme wastage and low stability. The results of the present work showed these drawbacks can be solved by cellulase immobilization on functionalized FeO magnetic nanoparticles (MNPs) with reactive red 120 (RR120) as the affinity ligands.
View Article and Find Full Text PDFNeuroethology of natural actions in freely moving monkeys.
Science
January 2025
Department of Medicine and Surgery, University of Parma, Parma, Italy.
The current understanding of primate natural action organization derives from laboratory experiments in restrained contexts (RCs) under the assumption that this knowledge generalizes to freely moving contexts (FMCs). In this work, we developed a neurobehavioral platform to enable wireless recording of the same premotor neurons in both RCs and FMCs. Neurons often encoded the same hand and mouth actions differently in RCs and FMCs.
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