Arginase 1 (ARG1) metabolizes arginine, thus reducing the availability of arginine as a substrate for nitric oxide synthase (NOS). The decreased production of nitric oxide (NO) by NOS and the production of ornithine by ARG1 affect immune responses and tissue regeneration at sites of infection, respectively. We here demonstrate that ARG1 is synthesized in myelocytes/metamyelocytes and is stored in gelatinase granules. In accordance with this, activated neutrophils coreleased ARG1 and gelatinase to the extracellular environment on stimulation with phorbol-12-myristate 13-acetate (PMA), formyl-methionyl-leucyl-phenylalanine (fMLP), or tumor necrosis factor alpha (TNF-alpha). Overall, these findings define ARG1 as a genuine gelatinase granule protein and support a model in which activated neutrophils release ARG1 at sites of infection to modulate immune responses and promote tissue regeneration.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1182/blood-2006-06-032599 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Periodontal ligament tissues support neutrophil differentiation and maturation processes.
Front Immunol
November 2024
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Article Synopsis
- The periodontal ligament is a crucial tissue that connects teeth to the surrounding bone, composed of various cell types important for its function, including poorly understood neutrophils.
- Researchers analyzed single-cell RNA sequencing to uncover the different populations of neutrophils in the periodontal ligament, identifying four distinct groups at various maturation stages.
- The findings suggest that the periodontal ligament acts as a supportive microenvironment for neutrophil development, indicating it plays a role in hematopoiesis and the maturation of these immune cells.
[ Granules inhibit autophagy and macrophage-mediated inflammatory response to improve acute exacerbations of chronic obstructive pulmonary disease in rats].
Nan Fang Yi Ke Da Xue Xue Bao
October 2024
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Ministry of Education, Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Objective: To investigate the inhibitory effect of Granules (TSG) on macrophage-mediated inflammatory response to alleviate acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in rats and explore the underlying mechanism.
Methods: Twenty-four rats were divided into control group, AECOPD model group, TSG treatment group, and moxifloxacin+salbutamol (MXF+STL) treatment group. In the rat models of COPD, AECOPD was induced by nasal instillation of on day 3 of week 9 after modeling, and saline, TSG or MXF+STL were administered via gavage on days 1 and 2 and days 4 to 7 of week 9.
[ inhibits proliferation, invasion, and migration and promotes apoptosis of non-small cell lung cancer cells].
Nan Fang Yi Ke Da Xue Xue Bao
October 2024
School of Pharmacy, Bengbu Medical University, Bengbu 233030, China.
Objective: To investigate the effect of on biological behaviors of non-small cell lung cancer (NSCLC) cells.
Methods: NSCLC cell lines PC-9 and A549 treated with different concentrations of preparations were examined for changes in proliferation, apoptosis, invasion and migration using CCK-8 assay, colony formation assay, flow cytometry, wound healing assay and Transwell assay. Western blotting was performed to detect the changes in protein expressions of Bax, Bcl-2, E-cadherin, vimentin, MMP2, and MMP9 in the treated cells.
Release of sputum neutrophil granules is associated with pulmonary function and disease severity in childhood asthma.
BMC Pulm Med
October 2024
Department of Pediatrics, Severance Hospital, Institute of Allergy, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-gu, Seoul, 03722, Korea.
Background: Myeloperoxidase (MPO) and human neutrophil lipocalin or neutrophil gelatinase-associated lipocalin (HNL/NGAL) are stored in neutrophil granulocytes and secreted upon activation of the cells. They have been proposed to reflect the degree of inflammation in the airways. However, their role as potential markers of disease severity in childhood asthma remains unknown.
View Article and Find Full Text PDFThe Miniaturized Isolation of Neutrophil Granules (MING) method allowed a deep proteome mapping of human neutrophil granules.
J Leukoc Biol
October 2024
Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
Neutrophils are the innate immune system's first line of defense, and their storage organelles are essential to their function. The storage organelles are divided into three different granule types named azurophilic, specific, and gelatinase granules, besides a fourth component called secretory vesicles. The isolation of neutrophil's granules is challenging, and the existing procedures rely on large sample volumes, about 400 mL of peripheral blood, precluding the use of multiple biological and technical replicates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!