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Quantifying metabolic activity of Ascaris suum L3 using resazurin reduction.
Parasit Vectors
July 2023
Institute of Immunology, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
Background: Helminth infections are an important public health problem in humans and have an even greater impact on domestic animal and livestock welfare. Current readouts for anthelmintic drug screening assays are stage development, migration, or motility that can be subjective, laborious, and low in throughput. The aim of this study was to apply and optimize a fluorometric technique using resazurin for evaluating changes in the metabolic activity of Ascaris suum third-stage larvae (L3), a parasite of high economic relevance in swine.
View Article and Find Full Text PDFA graphite furnace-atomic absorption spectrometry-based rubidium efflux assay for screening activators of the K 7.2/3 channel.
Arch Pharm (Weinheim)
May 2023
Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Greifswald, Germany.
For the characterization of K 7.2/3 channel activators, several analytical methods are available that vary in effort and cost. In addition to the technically elaborate patch-clamp method, which serves as a reference method, there exist several medium to high-throughput screening methods including a rubidium efflux flame-atomic absorption spectrometry (F-AAS) assay and a commercial thallium uptake fluorescence-based assay.
View Article and Find Full Text PDFA Polyclonal SELEX Aptamer Library Allows Differentiation of , and Cells from Human Dermal Fibroblasts.
J Fungi (Basel)
August 2022
Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Easy and reliable identification of pathogenic species such as yeasts, emerging as problematic microbes originating from the genus , is a task in the management and treatment of infections, especially in hospitals and other healthcare environments. Aptamers are seizing an already indispensable role in different sensing applications as binding entities with almost arbitrarily tunable specificities and optimizable affinities. Here, we describe a polyclonal SELEX library that not only can specifically recognize and fluorescently label cells, but is also capable to differentiate , and cells in flow-cytometry, fluorometric microtiter plate assays and fluorescence microscopy from human cells, exemplified here by human dermal fibroblasts.
View Article and Find Full Text PDFA versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation.
Protein Eng Des Sel
February 2021
Department of Biochemistry, University of Bayreuth, Universitätsstraße 30, 95447 Bayreuth, Germany.
Accumulation of plastic and subsequent microplastic is a major environmental challenge. With the discovery of potent polyethylene terephthalate (PET)-degrading enzymes, a new perspective arose for environmental decomposition as well as technical recycling. To explore the enormous diversity of potential PET-degrading enzymes in nature and also to conveniently employ techniques like protein engineering and directed evolution, a fast and reliable assay platform is needed.
View Article and Find Full Text PDFAntimicrobial and Efflux Pump Inhibitory Activity of Carvotacetones from Against Mycobacteria.
Antibiotics (Basel)
July 2020
Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
Carvotacetones () isolated from were screened for their antimycobacterial and efflux pump (EP) inhibitory potential against the mycobacterial model strains mc 155, ATCC 23366, and BCG ATCC 35734. The minimum inhibitory concentrations (MICs) of the carvotacetones were detected through high-throughput spot culture growth inhibition (HT-SPOTi) and microbroth dilution assays. In order to assess the potential of the compounds and to accumulate ethidium bromide (EtBr) in and , a microtiter plate-based fluorometric assay was used to determine efflux activity.
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